Application method and application of reagent for eliminating content of calcium ions in stigmas in overcoming selective fertilization of cruciferous plants

A cruciferous, selective fertilization technology, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of incompatible selfing, unsuitable promotion and low efficiency of distant hybridization, and achieve easy promotion. , Plant damage is small, the effect of simple and convenient operation

Pending Publication Date: 2022-01-11
SHANDONG AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many methods to overcome distant hybridization incompatibility and self-incompatibility that have been tried in production, but they all have the problem of low efficiency and are not suitable for promotion.

Method used

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  • Application method and application of reagent for eliminating content of calcium ions in stigmas in overcoming selective fertilization of cruciferous plants
  • Application method and application of reagent for eliminating content of calcium ions in stigmas in overcoming selective fertilization of cruciferous plants
  • Application method and application of reagent for eliminating content of calcium ions in stigmas in overcoming selective fertilization of cruciferous plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Chinese cabbage removes the flowers that have opened one day before spraying EDTA, and collects the seed bags. Carry out 2 times of EDTA solution spraying (EDTA solution contains 0.0125% Tween20, EDTA concentration is 0mM, 5mM, 8mM, 10mM) to new inflorescence in the morning of the next day, each interval is 30min, after the second spraying, self-flowering For pollen, the nesting belt prevents other pollen from being polluted. After 12 days, the fruit pods were dissected, and the affinity index was calculated.

[0044] The result is as Figure 2 to Figure 5 As shown in Table 1, spraying EDTA makes Chinese cabbage stigma Ca 2+ content was significantly reduced (for details, see figure 2 ), so that the average number of self-pollen tubes passing through each cabbage stigma increased from 0 to 6.64, 62.71, 127.79 (see image 3 ); Make the fruit pod length of 12 days after selfing of Chinese cabbage improve respectively from original 9.76mm to 23.21mm, 37.64mm, 41.68mm ...

Embodiment 2

[0048] Chinese cabbage removes the flowers that have opened one day before spraying EGTA, and collects the seed bags. Carry out 2 times of EGTA solution spraying (EGTA solution contains 0.0125% Tween20, EGTA concentration is 0mM, 5mM, 8mM, 10mM) to new inflorescence in the morning of the next day, every interval 30min, self-pollination after spraying for the second time , Harvesting belt to prevent pollution of other pollen. After 12 days, the fruit pods were dissected, and the affinity index was calculated.

[0049] After spraying EGTA solution, the Chinese cabbage stigma Ca 2+ content was significantly reduced (for details, see Figure 6 ), so that the average number of self-pollen tubes passing through each cabbage stigma increased from 0 to 13.19, 38.36, 23.20 (see Figure 7 ); Make the fruit pod length of 12 days after selfing of Chinese cabbage improve respectively from original 6.70mm to 41.27mm, 37.00mm, 43.41mm (for details see Figure 8 ); the affinity index of C...

Embodiment 3

[0053] Chinese cabbage removes the flowers that have opened one day before spraying EGTA, and collects the seed bags. Carry out 2 times of EGTA solution spraying (EGTA solution contains 0.0125% Tween20, EGTA concentration is 0mM, 5mM, 8mM) to new inflorescence emasculation in the morning of the next day, each interval is 30min, after the second spraying, pollen cabbage , Harvesting belt to prevent pollution of other pollen. After 12 days, the fruit pods were dissected, and the ovules in the fruit pods were counted.

[0054] The result is as Figure 10 ~ Figure 12 After spraying EGTA shown in Table 3, the average number of cabbage pollen tubes passing through each cabbage stigma was increased from 0 to 31.20, 105, 53.40 (for details see Figure 10 ); Make the fruit pod length of 12 days after the distant hybridization of Chinese cabbage and cabbage improve from original 0mm to 19.56mm, 18.79mm respectively (for details see Figure 11 ); after the distant hybridization of Chi...

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Abstract

The invention provides an application method and application of a reagent for eliminating the content of calcium ions in stigmas in selective fertilization of cruciferous plants, and relates to the technical field of cruciferous plant breeding. The application or the reagent is suitable for a self-incompatible line or a male sterile line with self-incompatibility of brassica crops. The method is easy and convenient to operate, easy to popularize, small in damage to plants, remarkable in effect of overcoming self-incompatibility, wide in application range and capable of being applied to brassica crops such as Chinese cabbages and cabbages and various radish crops; and in addition, the method can be further used for overcoming self-incompatibility of other cruciferous crops, and has important value for breeding and seed production of the cruciferous crops.

Description

technical field [0001] The invention belongs to the technical field of cruciferous plant breeding, and in particular relates to an application method and application of a reagent for removing calcium ion content in stigmas in overcoming selective fertilization of cruciferous plants. Background technique [0002] Self-incompatibility is a genetic mechanism evolved by angiosperms to prevent self-pollination and promote cross-pollination, which plays an important role in preventing self-depression and promoting offspring fitness. According to the source of male recognition factors, self-incompatibility can be divided into sporophytic self-incompatibility and gametophytic self-incompatibility. Cruciferous plants such as Chinese cabbage are typical sporophyte self-incompatibility, showing that self-pollen cannot germinate on the stigma, or entangle on the stigma papillae cells after germination and cannot enter the stigma, while heterogamic pollen can germinate , grow, enter the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
CPCA01H1/021
Inventor 段巧红黄家保崔晓爽王洁陈雨
Owner SHANDONG AGRICULTURAL UNIVERSITY
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