Application method and application of reagent for eliminating content of calcium ions in stigmas in overcoming selective fertilization of cruciferous plants
A cruciferous, selective fertilization technology, applied in the fields of botanical equipment and methods, application, plant genetic improvement, etc., can solve the problems of incompatible selfing, unsuitable promotion and low efficiency of distant hybridization, and achieve easy promotion. , Plant damage is small, the effect of simple and convenient operation
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Embodiment 1
[0043] Chinese cabbage removes the flowers that have opened one day before spraying EDTA, and collects the seed bags. Carry out 2 times of EDTA solution spraying (EDTA solution contains 0.0125% Tween20, EDTA concentration is 0mM, 5mM, 8mM, 10mM) to new inflorescence in the morning of the next day, each interval is 30min, after the second spraying, self-flowering For pollen, the nesting belt prevents other pollen from being polluted. After 12 days, the fruit pods were dissected, and the affinity index was calculated.
[0044] The result is as Figure 2 to Figure 5 As shown in Table 1, spraying EDTA makes Chinese cabbage stigma Ca 2+ content was significantly reduced (for details, see figure 2 ), so that the average number of self-pollen tubes passing through each cabbage stigma increased from 0 to 6.64, 62.71, 127.79 (see image 3 ); Make the fruit pod length of 12 days after selfing of Chinese cabbage improve respectively from original 9.76mm to 23.21mm, 37.64mm, 41.68mm ...
Embodiment 2
[0048] Chinese cabbage removes the flowers that have opened one day before spraying EGTA, and collects the seed bags. Carry out 2 times of EGTA solution spraying (EGTA solution contains 0.0125% Tween20, EGTA concentration is 0mM, 5mM, 8mM, 10mM) to new inflorescence in the morning of the next day, every interval 30min, self-pollination after spraying for the second time , Harvesting belt to prevent pollution of other pollen. After 12 days, the fruit pods were dissected, and the affinity index was calculated.
[0049] After spraying EGTA solution, the Chinese cabbage stigma Ca 2+ content was significantly reduced (for details, see Figure 6 ), so that the average number of self-pollen tubes passing through each cabbage stigma increased from 0 to 13.19, 38.36, 23.20 (see Figure 7 ); Make the fruit pod length of 12 days after selfing of Chinese cabbage improve respectively from original 6.70mm to 41.27mm, 37.00mm, 43.41mm (for details see Figure 8 ); the affinity index of C...
Embodiment 3
[0053] Chinese cabbage removes the flowers that have opened one day before spraying EGTA, and collects the seed bags. Carry out 2 times of EGTA solution spraying (EGTA solution contains 0.0125% Tween20, EGTA concentration is 0mM, 5mM, 8mM) to new inflorescence emasculation in the morning of the next day, each interval is 30min, after the second spraying, pollen cabbage , Harvesting belt to prevent pollution of other pollen. After 12 days, the fruit pods were dissected, and the ovules in the fruit pods were counted.
[0054] The result is as Figure 10 ~ Figure 12 After spraying EGTA shown in Table 3, the average number of cabbage pollen tubes passing through each cabbage stigma was increased from 0 to 31.20, 105, 53.40 (for details see Figure 10 ); Make the fruit pod length of 12 days after the distant hybridization of Chinese cabbage and cabbage improve from original 0mm to 19.56mm, 18.79mm respectively (for details see Figure 11 ); after the distant hybridization of Chi...
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