Method for rapidly realizing sweet potato transgenosis

Abstract

The invention discloses a method for rapidly realizing sweet potato transgenosis. The method comprises the following steps: injecting agrobacterium tumefaciens liquid containing a target gene expression vector into a first node, a second node or a third node of sweet potatoes in a 4-6 leaf stage, and then cultivating and detecting to obtain sweet potato transgenic plants or tissues containing the target gene. The invention provides the method for rapidly and efficiently realizing sweet potato transgenosis, and compared with traditional cross breeding and tissue culture, the method provided by the invention not only overcomes the problems of incompatibility of interspecific hybridization, low regeneration frequency, large difference in regeneration capacity between varieties and the like, but also greatly shortens the sweet potato genetic transformation and breeding period. By utilizing the method, a target gene or a specific DNA fragment can be introduced into a sweet potato tissue, a transformed sweet potato seedling can be quickly and efficiently obtained, the characteristics and resistance which are not possessed by the existing sweet potato variety are improved, and the sweet potato variety is greatly improved.

Description

Technical field: [0001] The invention relates to the field of plant technology, in particular to a method for rapidly realizing sweet potato transgene. Background technique: [0002] Sweet potato is an important economic crop and food crop in the world. China is the largest producer and consumer of sweet potato in the world. Cultivating new varieties of sweet potato with high yield, high resistance and high quality is the main direction to promote the development of sweet potato industry. At present, there are mainly two ways to obtain new varieties of sweet potato: traditional hybrid breeding and tissue culture. However, traditional hybrid breeding has a narrow genetic basis of sweet potato, close linkage between good genes and bad traits, incompatible interspecific hybridization, long breeding cycle, and some rare varieties. However, tissue culture has problems such as low plant regeneration frequency, long cycle, poor repeatability, and large differences in regeneration a...

AI Technical Summary

Problems solved by technology

At present, there are mainly two ways to obtain new varieties of sweet potato: traditional hybrid breeding and tissue culture. However, traditional hybrid breeding has narrow genetic basis of sweet potato, close linkage of good genes and bad traits, incompatibility of interspecific hybridization, long breeding cycle, and some rare varieties. However, tissue culture has problems such as low plant regeneration frequency, long cycle, poor repeatability, and large differences in regeneration ability among varieties, which seriously limit the process and efficiency of sweet potato breeding.

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