Method for prolonging storage time of Aenasius bambawalei Hayat and application thereof
A technology of Bancroft's jumping bee and storage time, which is applied in the field of low-temperature storage of insects, can solve the problems of prolonging the life of natural enemies, etc., and achieve the effect of improving low-temperature storage time, improving cold resistance, and prolonging the life of female bees
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Embodiment 1
[0025] Embodiment 1 Effect of different ages of low-temperature treatment on Leaperia bancroft on its eclosion rate
[0026] The total developmental period of Le. bancrofti at room temperature is 14.5 days, of which the egg, 1st instar larvae, 2nd instar larvae, 3rd instar larvae, 4th instar larvae, prepupa and pupae are 1.5d, 1d, 1d respectively , 2d, 2.5d, 1d and 5.5d. Therefore, the female adults of Pythococcus hibiscus were selected 1d, 2d, 3d, 5d, 8d, 9d and 12d after being parasitized by E. bancrofti, and they were connected to germinated potatoes, and then the potatoes with worms were placed in medical The storage test was carried out in a refrigerator, and the storage condition was 10 days at 4°C. There were 7 treatments in total, and 10 replicates for each treatment. The B. bancrofti that had not been stored in low temperature was used as the control of each treatment. After the end of the storage period, take out the fuso cotton mealybug (Pythias sinensis) and plac...
Embodiment 2
[0031] Embodiment 2 Effects of Low Temperature Acclimatization on the Eclosion of Leaperia bancroft Adults
[0032] Five different temperatures (12°C, 14°C, 16°C, 18°C and 20°C) and five different photoperiods (L8:D16, L10:D14, L12:D12, L14:D10 and L16:D8) were selected for the experiment , a total of 25 treatments were set up, and 30 female adults of Pythococcus hibiscus were placed in each treatment for 7 days after being parasitized by A. bancrofti. Then, put the above parasitized Pythococcus hibiscus into the light incubator with corresponding conditions for treatment, and observe and record the survival rate of Pythococcus hibiscus and the emergence of P. bancrofti every day until there is no Jump until the bee emerges. In the present invention, the judging method until the eclosion of B. bancrofti is as follows: after the parasitic wasp is parasitized, observe whether there are eclosion holes on the scorpion scales day by day, if no eclosion holes have been seen after...
Embodiment 3
[0040] Embodiment 3 Effects of low temperature and short light treatment at different times on the emergence of Leaperia bancroft
[0041] Collect the female adults of Pythococcus hibiscus 7 days after being parasitized by E. bancrofti, place them in an environment with a temperature of 14°C and a photoperiod of L8:D16, treat them for 2 days, 4 days, 6 days, 8 days and 10 days respectively, and then transfer them to room temperature Continue feeding in the environment, wherein the conditions of the normal temperature environment are room temperature 26-28° C., relative humidity 70%-80%, and photoperiod L16:D8. The experiment consisted of 5 treatments, each treatment was repeated 3 times, and 30 parasitized female adults of P. fuso were placed in each repetition. The parasitoids reared at normal temperature were used as the control.
[0042] Table 4 Effects of low temperature and short light treatment at different times on the emergence of Leapertia bancroft
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