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Shallow liquid layer quasi-static microalgae culture system and use method

A microalgae culture, quasi-static technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of water and energy consumption, insufficient light received by single cells, and low yield of algae cells, etc., to achieve Save the medium, improve the growth efficiency of microalgae, and realize the effect of heat exchange

Pending Publication Date: 2022-04-05
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the embodiment of the present application is to provide a shallow liquid layer quasi-static microalgae culture system and its use method to solve the technical problems of insufficient single cell light receiving, low yield of algae cells, and water and energy consumption in the related art

Method used

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  • Shallow liquid layer quasi-static microalgae culture system and use method
  • Shallow liquid layer quasi-static microalgae culture system and use method
  • Shallow liquid layer quasi-static microalgae culture system and use method

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Embodiment 1

[0069] refer to figure 1 , the embodiment of the present invention provides a shallow liquid layer quasi-static microalgae culture system, the system may include: a culture tank 1, an exchange column 2, a gas mixing device 5, a pipeline circulation drive structure 9, a temperature adjustment device and light supplement device6.

[0070] The culture tank 1 is used to accommodate microalgae and culture fluid; specifically, the culture tank 1 can be a horizontally placed cuboid container (the length, width and height are respectively 100 cm × 100 cm × 10 cm, which can be fixed by a support with a height of 100 cm) .

[0071] The exchange column 2 communicates with the culture tank 1 through a first connecting pipe 7, which is used to accommodate the culture solution and accelerate heat transfer and mass transfer through bubbling. The first connecting pipe 7 extends into the exchange column 2 There is a liquid level height adjusting member 71 at one end; the angle of the liquid ...

Embodiment 2

[0110] The culture system configuration and culture method are the same as in Example 1. Others remain unchanged, by adjusting the angle of rotation of the elbow at the end of the first connecting pipe 7, the liquid level depth in the adjustment culture tank 1 is 10mm, and the liquid filling volume is 20L (10L in the culture tank 1 and 10L in the exchange column 2 ), observe the culture results, collect the supernatant (18L) after the culture is over, and then collect the attached algae mud to obtain concentrated algae pulp (2L), and the final density of algae cells in the algae pulp reaches 32.5g L -1 , the cell density in the serum is 0.1g L -1 , so the equivalent cell volume density is 3.34g L 1 , with an areal yield of 15.2 g m -2 d -1 .

Embodiment 3

[0112] The culture system configuration and culture method are the same as in Example 1. Others remain unchanged, by adjusting the angle of rotation of the elbow at the end of the first connecting pipe 7, the liquid level depth in the adjustment culture tank 1 is 15mm, and the liquid volume is 25L (15L in the culture tank 1 and 10L in the exchange column 2 ), observe the culture results, collect the supernatant (23L) after the culture is over, and then collect the attached algae mud to obtain concentrated algae pulp (2L), and the final density of algae cells in the algae pulp reaches 30.7g L -1 , the cell density in the serum is 0.1g L -1 , so the equivalent cell volume density is 2.55g L -1 , with an areal yield of 14.05 g m -2 d -1 .

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Abstract

The invention discloses a shallow liquid layer quasi-static microalgae culture system and a use method, and the system comprises: a culture tank for accommodating microalgae and a culture solution; the exchange column is communicated with the culture tank through a first connecting pipe and used for containing a culture solution, and one end, extending into the exchange column, of the first connecting pipe is provided with a liquid level height adjusting piece; the pipeline circulation driving structure comprises a second connecting pipe, a third connecting pipe and a first pump, one end of the second connecting pipe is communicated with the exchange column, the other end of the second connecting pipe is communicated with one end of the third connecting pipe, the other end of the third connecting pipe is communicated with the culture tank, and the first pump is arranged on the second connecting pipe; the gas mixing device is communicated with the bottom of the exchange column through a fourth connecting pipe; the temperature adjusting device is used for adjusting the temperature of the culture solution in the exchange column; and the light supplementing device is positioned beside the culture tank and is used for supplementing light to the microalgae in the culture tank. The method has the characteristics of water saving and energy saving, and can be applied to various fields of industrial production and carbon neutralization of microalgae.

Description

technical field [0001] The present application relates to the field of microalgae biotechnology, in particular to a quasi-static shallow liquid layer microalgae cultivation system and its use method. Background technique [0002] Microalgae is a general term for a class of microorganisms that contain chlorophyll a and can perform photosynthesis. The cells usually contain high value-added products such as protein, oil, polyunsaturated fatty acids, polysaccharides, natural pigments, and vitamins. Therefore, they have extremely high value. They are used in food It has been widely used in , feed, cosmetics and other industries, and has potential application prospects in carbon neutral fields such as biological carbon sequestration, sewage treatment (pollution and carbon reduction), biofuel, bioplastic and biofertilizer production. [0003] At present, there are more than 20,000 types of microalgae known, but very few have been industrialized. The bottleneck restricting the micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/36C12M1/34C12M1/04C12M1/02C12M1/00C12R1/89
Inventor 章真张荣庆陈夏姚丽萍刘晓军
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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