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191 human microhaplotype locus genetic marker as well as amplification primer and application thereof

A genetic marker and haplotype technology, applied in the field of forensic genetics identification, can solve problems such as not being able to participate in identification

Active Publication Date: 2022-04-08
HEBEI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the actual prosecution, it is often encountered that the parents of the child die or cannot participate in the identification, and the identification of the grandparent-grandson relationship (only grandparents and grandchildren participate in the identification), half-sibling identification, identification of uncle and nephew, identification of aunt and nephew, and identification of aunt and nephew are often required. This type of identification belongs to the second-level identification of kinship, which is one of the difficulties in current forensic research.

Method used

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  • 191 human microhaplotype locus genetic marker as well as amplification primer and application thereof
  • 191 human microhaplotype locus genetic marker as well as amplification primer and application thereof
  • 191 human microhaplotype locus genetic marker as well as amplification primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening for micro-haplotype loci:

[0042] The screening of 191 micro-haplotype loci on 22 autosomes was obtained by using the population data of 103 Han Chinese in Beijing and 105 Han Chinese in southern China in the Thousand Genomes database, and screened according to the following specific conditions:

[0043] Primary screening:

[0044] (1) Remove recombination hot spots; (2) Remove SNPs with a heterozygosity less than 0.4; (3) There are 2-5 SNPs within 250 bp of DNA fragment length, and a total of 245,479 micro-haplotype loci were screened. And use phase to estimate the haplotype and haplotype frequency of the micro-haplotype locus.

[0045] Double screening:

[0046] Further screening of the micro-haplotype loci screened above:

[0047] (1) There are 3 to 5 SNPs within 200 bp of the DNA fragment length; (2) The number of effective alleles is greater than 2.5; (3) Check the sequence characteristics within ±200 bp upstream and downstream of the micro-haplotype ...

Embodiment 2

[0052] A group of human micro-haplotype loci screened in Example 1 includes the following 191 human micro-haplotype loci:

[0053]

[0054]

[0055]

[0056]

[0057]

[0058]

[0059]

[0060]

Embodiment 3

[0062] The sequences of 191 pairs of primers for amplifying 191 human micro-haplotype loci in Example 2 are as follows:

[0063]1) SEQ ID NO.1-2; 2) SEQ ID NO.3-4; 3) SEQ ID NO.5-6; 4) SEQ ID NO.7-8; 5) SEQ ID NO.9-10; 6 ) SEQ ID NO.11-12; 7) SEQ ID NO.13-14; 8) SEQ ID NO.15-16; 9) SEQ ID NO.17-18; 10) SEQ ID NO.19-20; 11) SEQ ID NO.21-22; 12) SEQ ID NO.23-24; 13) SEQ ID NO.25-26; 14) SEQ ID NO.27-28; 15) SEQ ID NO.29-30; 16) SEQ ID NO.29-30; 16) SEQ ID NO.25-26; ID NO.31-32; 17) SEQ ID NO.33-34; 18) SEQ ID NO.35-36; 19) SEQ ID NO.37-38; 20) SEQ ID NO.39-40; 21) SEQ ID NO .41-42; 22) SEQ ID NO.43-44; 23) SEQ ID NO.45-46; 24) SEQ ID NO.47-48; 25) SEQ ID NO.49-50; 26) SEQ ID NO. 51-52; 27) SEQ ID NO.53-54; 28) SEQ ID NO.55-56; 29) SEQ ID NO.57-58; 30) SEQ ID NO.59-60; 31) SEQ ID NO.61 -62; 32) SEQ ID NO.63-64; 33) SEQ ID NO.65-66; 34) SEQ ID NO.67-68; 35) SEQ ID NO.69-70; 36) SEQ ID NO.71- 72; 37) SEQ ID NO.73-74; 38) SEQ ID NO.75-76; 39) SEQ ID NO.77-78; 40) SEQ ID NO.79-80...

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Abstract

The invention relates to the technical field of forensic genetics identification, and particularly discloses a genetic marker of 191 human microhaplotype loci as well as an amplification primer and application thereof. The 191 human micro-haplotype locus genetic marker provided by the invention combines the advantages of STR and SNP, and has the advantages of short amplified fragment, low mutation rate, good polymorphism and no stutter peak. When the 191 human micro-haplotype locus genetic marker provided by the invention is used for genetic relationship identification, the two-layer genetic relationship identification between two individuals can be independently solved, the requirement that only two persons participate in identification in the secondary genetic relationship such as the secondary genetic relationship of the cassytha, the secondary genetic relationship of the arrowhead and the secondary genetic relationship of the unconsciousness and the secondary genetic relationship of the unconsciousness and the secondary genetic relationship of the unconsciousness and the secondary genetic relationship of the unconsciousness are met, and the The method has good repeatability and extremely high sensitivity and stability. In forensic medicine identification, the method has obvious anti-degradation capability, and has extremely high application value in forensic actual detection cases.

Description

technical field [0001] The invention relates to the technical field of forensic genetics identification, in particular to genetic markers of 191 human micro-haplotype loci, amplification primers and applications thereof. Background technique [0002] Short tandem repeats (short tandem repeat, STR) and single nucleotide polymorphisms (singlenucleotide polymorphisms, SNP) are commonly used genetic markers in forensic science. Due to its high polymorphism and wide distribution, STR has become the mainstream genetic marker for individual identification and kinship identification, and plays an irreplaceable role in actual case testing. Compared with STR, SNP has a lower mutation rate and shorter amplified fragments. It can also provide ancestry information and predict individual phenotype characteristics. It has more advantages in the analysis of degraded samples and the inference of geographic ancestry. Although both STR and SNP have played an important role in judicial practic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12N15/11C12Q1/6851C12Q1/6806
Inventor 李淑瑾丛斌杜情情卢朝龙马冠车付丽红王茜付光平
Owner HEBEI MEDICAL UNIVERSITY
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