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Development and Application of Specific Molecular Marker of Wheat Powdery Mildew Resistance Gene pm60 in Urartu

A technology for wheat powdery mildew and DNA molecules, which is applied in the fields of genetic engineering and molecular biology, and can solve problems such as limiting identification and assisted breeding.

Active Publication Date: 2020-12-04
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific marker of Pm60 gene has not been reported, which limits the identification of Pm60 gene in Urartu wheat and molecular marker-assisted breeding.

Method used

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  • Development and Application of Specific Molecular Marker of Wheat Powdery Mildew Resistance Gene pm60 in Urartu
  • Development and Application of Specific Molecular Marker of Wheat Powdery Mildew Resistance Gene pm60 in Urartu
  • Development and Application of Specific Molecular Marker of Wheat Powdery Mildew Resistance Gene pm60 in Urartu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Embodiment 1, identification of Uraltu wheat resistance spectrum

[0061] 1. Identification of powdery mildew resistance

[0062] 1. Use common wheat Xuezao to propagate powdery mildew;

[0063] 2. Plant Uraltu wheat seedlings to be identified in a sterile greenhouse. When the leaves grow to one leaf and one heart, inoculate 8 wheat powdery mildew physiological races E01, E05, E07, E09, E15, E18, E20, E13- 10. Use the inoculation tower to fully inoculate the wheat leaves with fresh spores of powdery mildew. Culture at 22°C, 16h light, 8h dark, humidity 70%.

[0064] The identification method refers to (Liu et al.1999), figure 1 It is a schematic diagram of the identification of powdery mildew resistance in Uraltu wheat, in which, note: 0 is immune; 0 is allergic necrosis; 1 is high resistance; 2 is moderate resistance; 3 is moderate; 4 The grade is high sense.

[0065] The resistance of 200 Urartu wheat germplasms to 8 wheat powdery mildew physiological races E01, ...

Embodiment 2

[0112] Embodiment 2, the application of Pm60 marker to judge the powdery mildew resistance of the wheat to be tested

[0113] 1. Identification of powdery mildew resistance

[0114] The cultivated einkorn wheat in Table 2 was identified using the powdery mildew resistance identification method in Example 1, and the results are shown in Table 2.

[0115] 2. Pm60 markers to judge the powdery mildew resistance of the tested wheat

[0116] 1. Utilize the CTAB method to extract the genomic DNA of the cultivated einkorn wheat in Table 2.

[0117] 2. PCR amplification

[0118] With the genomic DNA of the cultivated emmer wheat in Table 2 as a template, mark 1 (Pm60-F (3000T), Pm60-R (3465T)) and mark 2 (Pm60-F ( 2900T), Pm60-R(3465T)) for PCR amplification, as follows 1) or 2):

[0119] 1) The shown method comprises the steps:

[0120] Use Pm60-F (3000T) and Pm60-R (3465T) to amplify the DNA of the wheat to be tested, detect the PCR amplification product,

[0121] If the PCR am...

Embodiment 3

[0140] Example 3, Potential of Pm60-specific markers in molecular marker-assisted breeding in common wheat

[0141] Adopt the second method of embodiment 2 to common wheat Xuezao, Beijing 411, Shi 4185, Chinese spring, chancellor, Filder, Kenong 199, Lankao 86, Ermans wheat to 11 common wheats susceptible to the E13-10 bacterial strain at the seedling stage , Yangbai wheat and Zhengnong 17 failed to amplify any bands, indicating that these wheats are not powdery mildew resistant wheats containing the Pm60 gene, and the Pm60 gene and the developed Pm60 specific markers can be applied to these Disease resistance improvement of materials and molecular marker assisted breeding.

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Abstract

The invention discloses a development and an application of a triticumurartu anti-powdery mildew gene Pm60 specific molecular marker. The invention provides a primer set for identifying wheat powderymildew resistance. The primer set comprises a primer pair A composed of a primer 1 and a primer 2; the primer 1 is a single stranded DNA molecule shown as a sequence 2 in a sequence table; the primer2 is the single stranded DNA molecule shown as a sequence 3 in the sequence table; the primer set comprises a primer pair B composed of a primer 3 and a primer 2; the primer 3 is the single stranded DNA molecule shown as a sequence 1 in the sequence table. The molecular marker found by the invention has the advantages of great specificity, capability of accurately identifying Pm60a, Pm60b and Pm60genotypes with disease resistance in triticumurartu through a PCR method, short amplified fragments and suitability for application in molecular marker assistant breeding.

Description

technical field [0001] The invention belongs to the fields of genetic engineering and molecular biology, and in particular relates to the development and application of Uraltu wheat powdery mildew resistance gene Pm60 specific molecular marker. Background technique [0002] Wheat powdery mildew is one of the main diseases of wheat, which seriously threatens the production safety of wheat. Many of the existing powdery mildew resistance genes are from close relatives of wheat. In the 1970s, disease resistance breeding began to use a large number of Pm8 genes carried by the wheat-rye T1BL / 1RS translocation line, making wheat large The single disease resistance gene in the large-scale promotion varieties led to a rapid increase in the virulence frequency of the Pm8 gene in the wheat powdery mildew. One of the reasons for the epidemic of powdery mildew in my country from 1990 to 1991 was the loss of Pm8 gene disease resistance (Zhou Yang et al., 2004; Zhuang et al., 1993; Li Hon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 沈前华李映辉赵夫凯周立训
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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