Primer arm TaqMan-MGB probe PCR hepatitis b virus detection method and reagent box thereof
A hepatitis B virus and detection method technology, applied in biochemical equipment and methods, microbial measurement/inspection, fluorescence/phosphorescence, etc., can solve the problems of increasing design difficulty and hindering probe combination, so as to improve efficiency, Effect of improving detection sensitivity, typical temperature denaturation and renaturation characteristics
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[0029] Below in conjunction with specific embodiment, further elaborate the present invention in detail, the prominent substantive characteristic and positive effect of the present invention can be manifested from the following embodiment, but they are not to limit the present invention in any way.
[0030] Material:
[0031] (1) Bacterial strain: Escherichia coli DH5α was donated by the Department of Biochemistry, Xuzhou Medical College
[0032] (2) Reagents and solutions: Taq enzyme (Promega), vector pUC19, IPTG and X-gal, 100bp DNA Ladder Marker, PCR product recovery kit (Shanghai Gaming Company), T4 DNA ligase, restriction endonuclease BamH I And EcoR I (SibEnzyme Company), dNTP, TRIS, EDTA, Trizol, RNase Inhibitor, pUC19DNA / Mspl Marker, DEPC, tryptone for bacterial culture, yeast extract for bacterial culture (Shanghai Bioengineering Company), HBV DNA fluorescence quantification Detection Kit (Guangzhou Daan Company), Primer Synthesis (Shanghai Gaming Company), Probe Syn...
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