Preparation method of targeted delivery vaccine and erythrocyte vaccine

A technology for red blood cells and vaccines, applied in the field of vaccines, can solve problems such as the limitation of uptake efficiency of time-sensitive vaccines, and achieve the effects of preventing tumor recurrence and reducing inflammatory responses.

Pending Publication Date: 2022-05-06
初源汇企业管理(深圳)合伙企业(有限合伙)
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Problems solved by technology

[0002] The current vaccination methods are all subcutaneous injections. The main advantage is that the skin is the largest immune organ in the human body, and the subcutaneous tissue is rich in antigen-presenting cells, and the vaccination method is safe, simple and easy to use; but the antigen uptake depends on the antigen-presenting cells tending to the vaccination site , followed by ingestion of antigens and homing to lymph nodes or spleen to further activate T cells to play a role, timeliness and vaccine uptake efficiency are limited

Method used

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  • Preparation method of targeted delivery vaccine and erythrocyte vaccine
  • Preparation method of targeted delivery vaccine and erythrocyte vaccine
  • Preparation method of targeted delivery vaccine and erythrocyte vaccine

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Embodiment Construction

[0034] The present invention will be described in detail below with reference to the accompanying drawings and specific embodiments.

[0035] see figure 1 The present invention provides a method for preparing a targeted delivery vaccine, which can achieve efficient uptake of the prepared red blood cell vaccine by antigen presenting cells, including the following steps.

[0036] S1: Obtain the autologous peripheral blood of the experimental mice and store it in an anticoagulant blood collection tube; the peripheral blood adopts autologous peripheral blood, and the red blood cell vaccine made from the autologous peripheral blood can reduce the immune response.

[0037] S2: Use lymphocyte separation medium to separate red blood cells and lymphocytes from peripheral blood, and collect red blood cells;

[0038] S3: The collected erythrocytes were then washed with 0.01M PBS or 0.9% saline - centrifuged twice;

[0039] S4: Add 1xPBS (diluted with pure water) with a concentration ra...

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Abstract

The invention provides a preparation method of a targeted delivery vaccine and a red blood cell vaccine, which can realize timeliness and quicker and more efficient effect of uptake of the vaccine by antigen presenting cells, the antigen presenting cells are widely distributed in peripheral blood and various tissues, and main target organs are peripheral immune organs such as lymph nodes and spleens.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to a method for preparing a targeted delivery vaccine and a red blood cell vaccine. Background technique [0002] The current vaccination methods are all subcutaneous injections. The main advantage is that the skin is the largest immune organ of the human body, and the subcutaneous tissue has abundant antigen-presenting cells, and the vaccination method is safe and easy to use; however, antigen uptake depends on the tendency of antigen-presenting cells to the vaccination site. , followed by ingested antigens homing to lymph nodes or spleen to further activate T cells to play their role, with limitations in timeliness and vaccine uptake efficiency. [0003] Therefore, the prior art has shortcomings and needs to be improved. SUMMARY OF THE INVENTION [0004] The technical problem to be solved by the present invention is to provide a method for preparing a targeted delivery vaccine...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K39/00A61K47/46C12N5/078A61P37/02A61P35/00A61P31/04A61P31/12A61P29/00
CPCA61K9/5068A61K39/00A61K9/0019A61P37/02A61P35/00A61P31/04A61P31/12A61P29/00C12N5/0641A61K2039/515A61K2039/54A61K2039/575A61K2039/57C12N2509/00
Inventor 王飞吴松
Owner 初源汇企业管理(深圳)合伙企业(有限合伙)
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