Migration channel acquisition method and system for directional movement of cells

A technology of directional movement and acquisition method, which is applied in the field of acquisition of migration channels for directional movement of cells, which can solve the problems of short time and inability to induce cell migration, and achieve the effect of long induction time

Pending Publication Date: 2022-06-28
NINGBO FIRST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the directional migration of cells is mainly induced by biochemical factors. This induction method has obvious shortcomings, mainly reflected in: biochemical factors can not only target cells in a certain area, but also affect surrounding cells that do not need to be stimulated to migrate. impact; with the progress of cell life activities, biochemical factors will be metabolized and degraded, and the time for effectively inducing cell migration is short; in addition, biochemical factors cannot induce cell migration in three-dimensional space

Method used

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  • Migration channel acquisition method and system for directional movement of cells
  • Migration channel acquisition method and system for directional movement of cells
  • Migration channel acquisition method and system for directional movement of cells

Examples

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Embodiment 1

[0053] In order to increase the time to effectively induce cell migration, reduce the impact on other cells during the process of inducing cell migration, such as figure 1 As shown, the present invention proposes a migration channel acquisition method for cell directional movement, including:

[0054] S01: acquiring basic features of the cells to be migrated, the basic features including preset average length, average width and average height of the cells to be migrated;

[0055] This example is illustrated by inducing the directional migration of adherent cells M-22 human embryonic fibroblasts (hereinafter referred to as M-22).

[0056] First, obtain the basic characteristics of a preset M-22, and use imageJ or other image processing software to measure the average geometric size (rounded) of M-22 according to the observation results of the microscope, and determine the suitable M-22 cell growth according to the results of literature research. environment, the results are sh...

Embodiment 2

[0108] like figure 2 As shown, the present invention also provides a migration channel acquisition system for cell directional movement, including:

[0109] a cell feature acquisition module, used for acquiring basic features of the cells to be migrated, the basic features including preset average length, average width and average height of the cells to be migrated;

[0110] The channel feature acquisition module is used to set the straight line distance from the migration starting point to the migration end point of the cells to be migrated according to preset requirements, and obtain the minimum limit value of the turning radius in the migration channel according to the average length of the cells to be migrated. The average width and average height are obtained respectively to obtain the channel width and channel height of the migration channel;

[0111] The physical channel acquisition module is used to acquire preset physical migration channels with unequal turning radi...

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Abstract

The invention discloses a migration channel acquisition method and system for directional movement of cells, and relates to the field of directional movement of the cells, and the method comprises the following steps: acquiring a minimum limit value of a turning radius, a channel width and a channel height in a migration channel according to basic characteristics of to-be-migrated cells; obtaining a preset number of entity migration channels with unequal turning radiuses; obtaining the directed migration degree of the to-be-migrated cells in each entity migration channel by using each entity migration channel through the to-be-migrated cells; obtaining the entity migration channel with the directional migration degree greater than a preset threshold value as a preliminary screening migration channel, and migrating the to-be-migrated cells for a preset number of times by using each preliminary screening migration channel to obtain the average migration rate of each preliminary screening migration channel; the primary screening migration channel with the shortest migration duration is obtained through the average migration rate and serves as the target migration channel, and the problem that in the prior art, the time for inducing cell migration is short due to the fact that biochemical factors are metabolized and degraded in the induction process is solved.

Description

technical field [0001] The invention relates to the field of cell directional movement, in particular to a method and system for obtaining a migration channel for cell directional movement. Background technique [0002] Cell migration refers to the movement of cells from their original location to other locations. Cell migration plays an important role in life activities, such as: after skin injury, thrombocyte migrates to the injury site, and the directional movement of immune cells clears bacteria and viruses in the body, cancer. cell transfer, etc. Therefore, the induction of cell-directed migration is of great significance in the medical field. At present, the directional migration of cells is mainly induced by biochemical factors. This induction method has obvious shortcomings, which are mainly reflected in: biochemical factors cannot only target cells in a certain area, and can also affect the surrounding cells that do not need to stimulate migration. With the progre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12M3/00C12M1/36
CPCC12N5/0656C12M41/46
Inventor 黄瑜烨钱劲尹俊赖慧楠
Owner NINGBO FIRST HOSPITAL
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