Migration channel acquisition method and system for directional movement of cells
A technology of directional movement and acquisition method, which is applied in the field of acquisition of migration channels for directional movement of cells, which can solve the problems of short time and inability to induce cell migration, and achieve the effect of long induction time
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Embodiment 1
[0053] In order to increase the time to effectively induce cell migration, reduce the impact on other cells during the process of inducing cell migration, such as figure 1 As shown, the present invention proposes a migration channel acquisition method for cell directional movement, including:
[0054] S01: acquiring basic features of the cells to be migrated, the basic features including preset average length, average width and average height of the cells to be migrated;
[0055] This example is illustrated by inducing the directional migration of adherent cells M-22 human embryonic fibroblasts (hereinafter referred to as M-22).
[0056] First, obtain the basic characteristics of a preset M-22, and use imageJ or other image processing software to measure the average geometric size (rounded) of M-22 according to the observation results of the microscope, and determine the suitable M-22 cell growth according to the results of literature research. environment, the results are sh...
Embodiment 2
[0108] like figure 2 As shown, the present invention also provides a migration channel acquisition system for cell directional movement, including:
[0109] a cell feature acquisition module, used for acquiring basic features of the cells to be migrated, the basic features including preset average length, average width and average height of the cells to be migrated;
[0110] The channel feature acquisition module is used to set the straight line distance from the migration starting point to the migration end point of the cells to be migrated according to preset requirements, and obtain the minimum limit value of the turning radius in the migration channel according to the average length of the cells to be migrated. The average width and average height are obtained respectively to obtain the channel width and channel height of the migration channel;
[0111] The physical channel acquisition module is used to acquire preset physical migration channels with unequal turning radi...
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