Hyaluronic acid composite gel for filling lacrimal duct and preparation method of hyaluronic acid composite gel

A composite gel and hyaluronic acid technology, applied in prosthetics, medical science, etc., can solve the problems of unspecified molecular weight of hyaluronic acid, complicated preparation process, difficult injection, etc., to avoid Tyndall effect and high safety , the effect of supplementing skin nutrition

Pending Publication Date: 2022-08-09
HYAMED BIOTECHNOLOGY (ZHUHAI) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of this patent is complicated, and additional steps are required to remove the cross-linking agent, and the molecular weight of hyaluronic acid is not specified, so there are inevitably problems such as difficulty in injection and excessive metabolism

Method used

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  • Hyaluronic acid composite gel for filling lacrimal duct and preparation method of hyaluronic acid composite gel
  • Hyaluronic acid composite gel for filling lacrimal duct and preparation method of hyaluronic acid composite gel
  • Hyaluronic acid composite gel for filling lacrimal duct and preparation method of hyaluronic acid composite gel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Take 0.16g of sodium hyaluronate with a molecular weight of 1200kDa, 0.16g of sodium hyaluronate with a molecular weight of 250kDa and 0.05g of recombinant collagen with a molecular weight of 50kDa, mix and disperse them in phosphate buffered saline (PBS) to a total weight of 10g, stir Dissolve, measure the pH=5.5 of the mixed solution at the same time, add 0.1mol / L sodium hydroxide solution to adjust the pH of the mixed solution to 7, fully dissolve to obtain a clear homogeneous viscous solution, stir and degas in a vacuum mixer, the vacuum degree - 1000mbar, rotating speed 20rpm, stirring for 40 minutes, after degassing until a bubble-free viscous solution is obtained, the degassed viscous solution is filled into a pre-filled syringe under negative pressure and aseptic conditions, and the mixture is heated with steam and air at 110kPa. medium, so that the prefilled syringe after filling reaches 125°C within 8 minutes, and the temperature is maintained for 8 minutes.

Embodiment 2

[0034] Take 0.2 g of sodium hyaluronate with a molecular weight of 1300 kDa, 0.2 g of sodium hyaluronate with a molecular weight of 500 kDa and 0.07 g of recombinant collagen with a molecular weight of 60 kDa, mix and disperse them into phosphate buffered saline (PBS) to a total weight of 10 g, and stir. Dissolve, measure the pH of the mixed solution at the same time = 8.2, add 0.1mol / L hydrochloric acid solution to adjust the pH of the mixed solution to 8, fully dissolve to obtain a clear homogeneous viscous solution, stir and degas in a vacuum mixer, the vacuum degree is -1200mbar, The rotating speed is 20rpm, stirring for 50 minutes, after degassing until a bubble-free viscous solution is obtained, the degassed viscous solution is filled into a pre-filled syringe under negative pressure and aseptic conditions, and the steam-air mixed medium is heated at 110kPa, The filled pre-filled syringe was allowed to reach 130° C. within 10 minutes, and the temperature was maintained fo...

Embodiment 3

[0036] Take 0.12g of sodium hyaluronate with a molecular weight of 1000kDa, 0.12g of sodium hyaluronate with a molecular weight of 200kDa and 0.04g of recombinant collagen with a molecular weight of 40kDa, mix and disperse them into phosphate buffered saline (PBS) to a total weight of 10g, and stir. Dissolve, measure the pH=5.2 of the mixed solution at the same time, add 0.1mol / L sodium hydroxide solution to adjust the pH of the mixed solution to 6, fully dissolve and evenly obtain a clear homogeneous viscous solution, stir and degas in a vacuum mixer, the vacuum degree - 900mbar, rotating speed 20rpm, stirring for 30 minutes, after degassing to obtain a viscous solution without bubbles, filling the degassed viscous solution into a pre-filled syringe under negative pressure and aseptic conditions, heating steam-air mixing at 110kPa medium, so that the prefilled syringe after filling reaches 110 °C within 5 minutes, and the temperature is maintained for 5 minutes, and then the p...

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Abstract

The invention provides hyaluronic acid composite gel for filling lacrimal ducts and a preparation process of the hyaluronic acid composite gel. The composite gel is prepared by crosslinking medium-molecular-weight sodium hyaluronate, low-molecular-weight sodium hyaluronate and recombinant collagen, wherein the mass percent of the medium molecular weight sodium hyaluronate and the mass percent of the low molecular weight sodium hyaluronate in the composite gel are both 1.2-2.0%, the mass percent of the recombinant collagen in the composite gel is 0.4-0.7%, and the balance of the composite gel is the phosphate buffer solution. According to the preparation method, crosslinking is carried out through physical means of rapid heating, heat preservation and rapid cooling. According to the composite gel disclosed by the invention, the medium-molecular-weight sodium hyaluronate, the low-molecular-weight sodium hyaluronate and the recombinant collagen are matched, so that the filling effect can be ensured, the maintaining time of the filling effect can be prolonged, the Tyndall effect can be effectively avoided, the safety is high, and skin nutrition can be repaired and supplemented; the physical crosslinking method of rapid heating-heat preservation-rapid cooling does not need a crosslinking agent and has no residual risk.

Description

technical field [0001] The invention relates to the technical field of polymer materials, in particular to a preparation method of a hyaluronic acid composite gel and the prepared hyaluronic acid composite gel. Background technique [0002] With the improvement of living standards, people's pursuit of "beauty" has gradually increased, and filler products for facial and neck wrinkles have become the darling of the market. At present, the products used for tear trough filling and repairing on the market mainly include cross-linked sodium hyaluronate gel and sodium hyaluronate compound solution containing nutrients such as amino acids. Among them, cross-linked sodium hyaluronate gel has certain risks in tear trough filling. Due to the thin skin at the injection site, the requirements for the doctor's injection technique are very high. Inaccurate method or unclear anatomical structure of the tear groove will lead to poor injection effect. If the injection is too shallow, the te...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08L5/08C08L89/00A61L27/26A61L27/50A61L27/52A61L27/58
CPCC08J3/246A61L27/26A61L27/52A61L27/50A61L27/58C08J2305/08C08J2489/00A61L2400/06A61L2430/34C08L5/08C08L89/00
Inventor 张彦军王师亮罗玉娜王江田
Owner HYAMED BIOTECHNOLOGY (ZHUHAI) CO LTD
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