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Culture medium and method for tissue culture and quick-growth of Pachystachya lutea

A technology of tissue culture rapid propagation and culture medium, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of poor multiplication ability, loss of multiplication and growth ability, complicated cultivation process, etc., and achieve simple cultivation process Easy to grow, proliferate and grow vigorously, and have high survival rate after transplanting

Inactive Publication Date: 2006-01-11
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, although there is a report on the method of tissue culture and rapid propagation of golden bract flower, its cultivation process is complicated, especially in the cultivation process (generally after 6 to 8 generations), the petiole of the aseptic seedling is prone to abscission, and leaves fall. Degeneration makes sterile seedlings unable to carry out photosynthesis, loses the ability to continue to proliferate and grow, or has poor proliferative ability, small multiplication coefficient, and low survival rate of transplanted test tube seedlings

Method used

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  • Culture medium and method for tissue culture and quick-growth of Pachystachya lutea
  • Culture medium and method for tissue culture and quick-growth of Pachystachya lutea
  • Culture medium and method for tissue culture and quick-growth of Pachystachya lutea

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Embodiment 1

[0019] Embodiment 1. the preparation of golden bud flower tissue culture rapid propagation medium

[0020] Dissolve in distilled water to prepare culture medium according to the proportion of the types and masses of the following substances contained in each liter:

[0021] Ammonium Nitrate 750mg, Potassium Nitrate 900mg, Calcium Chloride Dihydrate 200mg, Magnesium Sulfate Heptahydrate 370mg, Potassium Dihydrogen Phosphate 90mg, Disodium EDTA 49.8mg, Ferrous Sulfate 37.1mg, Tetrahydrate Manganese sulfate 12 mg, zinc sulfate heptahydrate 4.5 mg, boric acid 6.2 mg, potassium iodide 0.83 mg, sodium molybdate dihydrate 0.25 mg, copper sulfate pentahydrate 0.025 mg, cobalt chloride hexahydrate 0.025 mg, thiamine hydrochloride 0.15 mg, Niacin 0.5 mg, pyridoxine hydrochloride 0.5 mg, inositol 50 mg, glycine 2 mg, ascorbic acid 50 mg, sucrose 35 g, carrageenan 6.5 g, and 6-benzyl purine 0.01 mg, indole acetic acid 0.08 mg, naphthalene Add 0.08 mg of acetic acid and 0.25 mg of paclobu...

Embodiment 2

[0022] Embodiment 2. the tissue culture rapid multiplication of golden bud flower

[0023] The golden bud flower tissue culture rapid propagation medium of embodiment 1 is put into 150mL Erlenmeyer flask, every bottle 50mL, through 120~125 ℃, 1.1kg / cm 2 High temperature and high pressure disinfection,

[0024] Use the axillary bud stems of adult golden buds as explants, disinfect with 70% alcohol for 45 to 60 seconds, then disinfect with 0.15% mercury chloride solution for 3 to 5 minutes, and rinse with sterile water for 4 to 5 minutes. Second-rate,

[0025] Inoculate the sterilized explants in a triangular flask containing culture medium under aseptic conditions on the ultra-clean workbench, and place it in a fluorescent lamp as a light source with a light intensity of 2000-2500lx for 14 hours a day , in a culture room with a temperature of 25-28°C when there is light, 20-23°C when it is dark, and a humidity of 60%, the first generation grows a root system in about 20 days,...

Embodiment 3

[0027] Embodiment 3. the preparation of golden bud flower tissue culture rapid propagation medium

[0028] Dissolve in distilled water to prepare culture medium according to the proportion of the types and masses of the following substances contained in each liter:

[0029] Ammonium Nitrate 800mg, Potassium Nitrate 800mg, Calcium Chloride Dihydrate 220mg, Magnesium Sulfate Heptahydrate 370mg, Potassium Dihydrogen Phosphate 80mg, Disodium EDTA 49.8mg, Ferrous Sulfate 37.1mg, Tetrahydrate Manganese sulfate 12 mg, zinc sulfate heptahydrate 4.5 mg, boric acid 6.2 mg, potassium iodide 0.83 mg, sodium molybdate dihydrate 0.25 mg, copper sulfate pentahydrate 0.025 mg, cobalt chloride hexahydrate 0.025 mg, thiamine hydrochloride 0.15 mg, Niacin 0.5 mg, pyridoxine hydrochloride 0.5 mg, inositol 50 mg, glycine 2 mg, ascorbic acid 20 mg, sucrose 35 g, carrageenan 6.5 g, and 6-benzyl purine 0.035 mg, indole acetic acid 0.12 mg, naphthalene Add 0.03 mg of acetic acid and 0.1 mg of paclobu...

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Abstract

A solid culture medium for high-speed tissue culture of golden-bud flower is prepared from 23 raw materials including ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, etc through proportionally mixing, regulating pH=5.8-6.0, and high-temp and pressure sterilizing. Its tissue culture method includes inoculating the explant which is the stem of axillary bud of adult golden-bud flower plant into said culture medium in glass bottle, culturing for 40 days, shearing the axillary bud's stem, repeating said steps for secondary culture for 30 days, and transplanting. Its advantage is high survival rate up to 98% or more.

Description

technical field [0001] The invention relates to a tissue culture rapid propagation medium and a tissue culture rapid propagation method for plants, in particular to a tissue culture rapid propagation medium and a tissue culture rapid propagation method for golden bracts. Background technique [0002] In the prior art, although there is a report on the method of tissue culture and rapid propagation of golden bract flower, its cultivation process is complicated, especially in the cultivation process (generally after 6 to 8 generations), the petiole of the aseptic seedling is prone to abscission, and leaves fall. Degeneration makes sterile seedlings unable to carry out photosynthesis, loses the ability to continue to proliferate and grow, or has poor proliferative ability, small multiplication coefficient, and low survival rate of test-tube seedlings transplanted. Contents of the invention [0003] The object of the present invention is to provide a kind of culture medium for...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 蒋泽平梁珍海李玉巧吴纲李雪萍李劲刘根林季永华吴礼才杨勇袁芳
Owner JIANGSU ACAD OF FORESTRY
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