A kind of human normal renal tubule primary cell and its in vitro isolation culture and application

Abstract

The invention discloses a primary human normal renal tubule cell and its in vitro isolation, culture and application. The cell is named primary human normal renal tubular cell XHRN‑01, and the preservation number is CCTCC NO: C201970. The primary cell isolation and culture method is as follows: Obtain in vitro the tissue adjacent to the renal cancer after clinical surgery, no cancer cell invasion is detected by pathology, blood vessels and adipose tissue in the tissue are removed, the tissue sample is digested with collagenase / trypsin, filtered, The cell pellet was obtained by low-speed centrifugation. After the erythrocytes were lysed by the erythrocyte lysate, the cells were resuspended with COMR complete medium, and the cell line was constructed by subculture. The cells of the present invention can be used in the physiological research of normal human cells, the drug toxicity research of normal cells in vitro, the pathogenesis of kidney and related diseases including renal cancer, chronic nephritis and infectious diseases, and the application of drug screening research.

Description

technical field [0001] The invention belongs to the field of cell biology, and more specifically relates to a primary human normal renal tubule cell and its in vitro isolation and application. Background technique [0002] The kidney is a functional organ with a large blood flow in the body. Toxic substances in the blood can quickly reach the kidney. After the drug enters the body, most drugs are filtered by the glomerulus, secreted by the proximal convoluted tubule, reabsorbed by the distal convoluted tubule, and the epithelial cells of the small closure. Metabolic processes such as degradation are excreted from the body, and this process can involve structural or functional changes in the kidneys, resulting in nephrotoxicity or renal injury. In addition, the kidney's function of concentrating urine further increases the concentration of toxic substances in kidney cells and renal tubules; at the same time, because the kidney tissue is in a state of high metabolism, requires...

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Problems solved by technology

[0006] The Chinese patent CN109022350A has been published in the prior art, relying on high-purity primary renal tubular epithelial cells of mice to establish a cell model, although the purity of the primary renal tubular epithelial cells obtained in the experiment is as high as 92%, but it is used in the study of acute kidney injury or chronic renal failure The damage mechanism of renal tubular epithelial cells in diseased renal tubular epithelial cells, there are still species differences between primary renal tubular epithelial cells of mice and humans, which cannot accurately reflect the clinical results

[0007] The literature published in the prior art "Research on Primary Culture, Passage and Identification of Human Kidney Proximal Tubular Epithelial Cells" provides a method for the isolation and culture of human renal tubular epithelial cells to obtain human renal tubular epithelial cells, excluding species However, the number of subcultures of human renal tubular epithelial cells is limited, the number of progeny cells obtained is limited, and the morphology of cells obtained by continuous culture changes to senescence and death, which is not conducive to the study of drug cytotoxicity and nephrotoxicity

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