Extraction method of autologous adipose-derived stem cells for scar repair

An extraction method and technology of fat source, which is applied in the field of extraction of autologous fat-derived stem cells, can solve problems such as not necessarily good results, expensive products, and many chemical components, so as to achieve vigorous proliferation, speed up repair, and improve cell activity. Effect

Inactive Publication Date: 2021-04-13
上海南滨江细胞生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies of the prior art, the present invention provides a method for extracting autologous adipose-derived stem cells for scar repair, which is used to solve the problem of repair cream, repair liquid, repair paste, etc. The restoration products in different degrees have the problems of excessive chemical components, slow effect, expensive products, and the results are not necessarily very good.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for extracting autologous adipose-derived stem cells for scar repair, specifically comprising the following steps:

[0025] Step S1: Select a part of the patient's body as the part to extract fat and mark it. Use local anesthesia and swelling solution to treat the part. After the local anesthesia and swelling are treated, use a 50mL syringe and a 4mm liposuction needle to insert subcutaneously along the direction of the skin incision For adipose tissue, absorb 50mL of adipose tissue fluid;

[0026] Step S2: Place the syringe with adipose tissue fluid at 4°C in 500 mL of normal saline, let it stand for 10 minutes, drain the bottom layer, then transfer the remaining liquid to a 50 mL centrifuge tube, place it in a refrigerated high-speed centrifuge, and Centrifuge for 5 minutes at a temperature of 4°C and a rotational speed of 1000 r / min. After the centrifugation, take the upper layer to obtain fat particles;

[0027] Step S3: Put the fat particles obtained in s...

Embodiment 2

[0031] A method for extracting autologous adipose-derived stem cells for scar repair, specifically comprising the following steps:

[0032] Step S1: Select a part of the patient's body as the part to extract fat and mark it. Use local anesthesia and swelling solution to treat the part. After the local anesthesia and swelling are treated, use a 50mL syringe and a 4mm liposuction needle to insert subcutaneously along the direction of the skin incision For adipose tissue, absorb 50mL of adipose tissue fluid;

[0033] Step S2: Place the syringe with adipose tissue fluid at 5°C in 500mL of normal saline, let it stand for 12.5min, drain the bottom layer, then transfer the remaining liquid to a 50mL centrifuge tube, place in a refrigerated high-speed centrifuge, Centrifuge for 5 minutes at a temperature of 4°C and a rotational speed of 1100 r / min. After the centrifugation, take the upper layer to obtain fat particles;

[0034] Step S3: Put the fat particles obtained in step S2 into ...

Embodiment 3

[0038] A method for extracting autologous adipose-derived stem cells for scar repair, specifically comprising the following steps:

[0039] Step S1: Select a part of the patient's body as the part to extract fat and mark it. Use local anesthesia and swelling solution to treat the part. After the local anesthesia and swelling are treated, use a 50mL syringe and a 4mm liposuction needle to insert subcutaneously along the direction of the skin incision For adipose tissue, absorb 50mL of adipose tissue fluid;

[0040] Step S2: Place the syringe with adipose tissue fluid at 6°C in 500 mL of normal saline, let it stand for 15 minutes, drain the bottom layer, then transfer the remaining liquid to a 50 mL centrifuge tube, place it in a refrigerated high-speed centrifuge, and Centrifuge for 5 minutes at a temperature of 4°C and a rotational speed of 1200 r / min. After the centrifugation, take the upper layer to obtain fat particles;

[0041] Step S3: Put the fat particles obtained in s...

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PUM

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Abstract

The invention discloses an extraction method of autologous adipose-derived stem cells for scar repair. The extraction method of autologous adipose-derived stem cells for scar repair comprises the following steps: collecting adipose tissue fluid, separating adipose particles, crushing the adipose particles, performing primary culture, performing subculture and the like. The extracted autologous adipose-derived stem cells have good differentiation and proliferation capacity, can promote division and renewal of autologous adipose cells of an organism and accelerate repair of facial scars, enable the cells to keep a good growth state and vigorous proliferation activity, meet the requirement of scar treatment, and have no side effect in the proliferation and repair process of autologous cells of the organism. The problems that at the present stage, modes such as repairing paste, repairing liquid and repairing patches are usually adopted for scar repairing, and most repairing products have too many chemical components to different extents, take effect slowly, are high in product price and have no good effect, are effectively solved.

Description

technical field [0001] The invention relates to the technical field of scar repair, in particular to a method for extracting autologous adipose-derived stem cells for scar repair. Background technique [0002] Autologous stem cells are a kind of pluripotent cells with self-replication ability. Under certain conditions, they can differentiate into a variety of APSC pluripotent cells. They are a kind of primitive cells with self-replication and multi-directional differentiation potential. Autologous stem cells are not fully differentiated and immature cells. They have the potential to regenerate various tissues and organs and the human body, and have changed the way to deal with human diseases. They are called "universal cells" in the medical field. Autologous stem cells are a kind of "seed cells". The animal body realizes cell renewal through the division of autologous stem cells, so as to ensure the continuous growth and development of the animal body. When encountering a d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775A61K35/28A61P17/02
CPCC12N5/0667A61K35/28A61P17/02C12N2509/00C12N2509/10
Inventor 占震锋李庆静
Owner 上海南滨江细胞生物科技有限公司
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