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Vaccine-induced hepatitis B viral strain and uses thereof

A hepatitis B virus, wild-type technology, applied in the direction of virus, antiviral agent, sensory disease, etc., can solve the problem of virus transmission

Inactive Publication Date: 2006-05-10
GOVERNMENT OF THE REPUBLIC OF SINGAPORE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The presence of HBsAg is used as the primary detection marker for HBV carriers who may be at risk of transmitting the virus

Method used

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  • Vaccine-induced hepatitis B viral strain and uses thereof
  • Vaccine-induced hepatitis B viral strain and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0110] The nucleotide sequence and induced amino acid sequence of the hepatitis B virus with a vaccine-induced mutation (changing glycine to arginine) at amino acid residue 145 of the major surface antigen was determined as follows:

[0111] 1. Isolation of Viral DNA

[0112] Viral DNA was isolated from a serum sample (20 / 815c) obtained from an 11-year-old Chinese child born to a mother carrying wild-type surface antigen. Despite a negative test result at birth, the child received a combination of HBIG and a plasma-derived vaccine. He was tested positive for hepatitis B virus surface antigen one year later, and sequence analysis of the "a" determinant revealed a vaccine-induced mutation at amino acid residue 145 of the major antigen.

[0113] The separation method used was:

[0114] Add 400ul of dissipating buffer (Tris-Cl 10mM, pH7.4, EDTA 1mM, and sodium lauryl sulfate 2%) and 25ul of proteinase K (20mg / ml) to 200ul of serum samples, and incubate at 65°C for 3 Hour. Vira...

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PUM

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Abstract

The present invention provides a hepatitis B virus isolate named as the surface antigen of human hepatitis B virus-'S'-145 Singapore strain (change glycine to arginine), and the virus genome composed of it has been obtained in December, 1997 It was preserved in the European Cell Culture Collection on the 15th, with the registration numbers P97121504, P97121505 and P97121506. The present invention also provides isolated nucleic acids and purified peptides encoding a polypeptide, wherein said polypeptide is a mutant major surface antigen of a hepatitis B virus strain, said polypeptide having an amino acid sequence similar to that of wild-type hepatitis B virus The amino acid sequence of the major surface antigen of said polypeptide differs in that the amino acid at position 145 of said polypeptide is arginine instead of glycine. The present invention further provides an isolated nucleic acid encoding a peptide encoded by a nucleic acid molecule comprising nucleotides 527 to 595 of SEQ ID NO:1, and purified peptides thereof. The invention also provides various methods of using the disclosed isolated nucleic acids and polypeptides. The present invention also provides various applications of said virus strain and its protein.

Description

[0001] Throughout the application, various references are given in parentheses. The disclosures of these publications in their entirety are hereby incorporated by reference herein to more fully describe the state of the art to which this invention pertains. Background of the invention [0002] The present invention relates to the hepatitis B virus genome with a vaccine-induced mutation (changing glycine to arginine) at the 145th amino acid residue in the main surface antigen, its nucleotide sequence, and four main protein sequences induced , antigens, antibodies, detection systems, development of effective vaccines and antiviral reagents. [0003] First discovered in 1963, hepatitis B virus is a parenterally transmitted human virus. Although these viruses are not significantly cytotoxic and do not cause massive cell death, they are the cause of a major infectious disease affecting both adults and children worldwide. The presence of HBsAg is used as the primary detection mark...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/51C12N7/01C07K14/02C07K16/08C12Q1/68A61K39/29C12N15/09A61K38/00A61K38/17A61K38/22A61K39/00A61K45/00A61K48/00A61P1/16A61P9/00A61P11/06A61P13/12A61P17/00A61P17/02A61P19/02A61P27/02A61P29/00A61P31/00A61P31/14A61P31/20A61P35/00A61P43/00C07K14/71C07K16/00C07K19/00C12N1/19C12N1/21C12N5/10C12N7/00C12N15/12C12N15/62C12P21/02C12P21/08C12Q1/70G01N33/53G01N33/566G01N33/576
CPCC07K14/005C12N7/00C12N2730/10122C12Q1/706G01N33/5764C07K14/71C07K2319/00C07K2319/30A61K39/29C12N2730/10134A61K39/12A61P1/16A61P11/06A61P13/12A61P17/00A61P17/02A61P19/02A61P27/02A61P29/00A61P31/00A61P31/14A61P31/20A61P35/00A61P43/00A61P9/00
Inventor 温崇仁林玉娇梁爱玲赵奕陈维宁
Owner GOVERNMENT OF THE REPUBLIC OF SINGAPORE
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