Prodn. and use of novel fungitype insecticide

A fungal pesticide and a new type of technology, applied in the field of the entomopathogenic fungus Nomura reinhardtii, can solve the problems of lagging research on the efficient application of microbial pesticides, excessive pesticide residues, and unstable control effects, achieving sustainable control, overcoming Application bottleneck, the effect of speeding up the practical process

Inactive Publication Date: 2007-06-06
JIANGSU LIXIAHE REGION AGRI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problem of excessive pesticide residues in agricultural products from the source, realize the high-quality and safe production of agricultural products, ensure the health of the people and earn foreign exchange from agricultural products, carry out research and development of new biological source pesticides such as fungi and viruses, and establish a wide Spectrum, high-efficiency, and insect disease-prevalent biological control platform, with the key tasks of overcoming the weaknesses of microbial pesticides such as narrow insecticidal spectrum, slow action speed, and unstable control effect, and lagging behind in the research of efficient application technology of microbial pesticides, to provide a Production and Application of New Type Fungal Insecticides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Culture characteristics of Nomura strains

[0014] 1. The effect of temperature on the growth of bacterial strains

[0015] All Nomura strains can grow at 14-34°C, and grow fastest at 26-30°C. Conidia can be seen 2 days after inoculation. The optimum temperature is 30°C; below 14°C, growth slows down significantly above 34°C. The spore production of the bacteria is also the highest at 30°C (see Table 1). The growth rate and sporulation of the 6 strains were inconsistent, and the sporulation of Nr04-3 was significantly higher than that of other strains after 10 days of inoculation.

[0016]

[0017] Note: The same letter in the same column in the table means that the difference is not significant at the 0.05 level.

[0018] 2. The effect of pH on the growth of strains

[0019] Nomura strains can grow in the pH range of 2 to 9, and grow slowly when it is lower than 3 or higher than 8. The optimum pH is 5 to 7, and the growth is best when pH=6, and the spore ...

Embodiment 2

[0027] Media Screening Test

[0028] 1. Effects of different culture media on the sporulation traits of strains

[0029] The morphological changes of the same strain were not significant on PDA, organic nitrogen and SDAY medium. It is easier to obtain a large number of conidia with organic nitrogen medium and SDAY medium. On organic nitrogen medium and SDAY, the spores of Nomura reyzii appeared, and the spore production was generally 1 day earlier than that on the PDA plate, and the spore production was larger than that on the PDA plate. The ratio of organic nitrogen medium to PDA was 1.17-8.75, and SDAY The ratio is 1.09-10. The optimum harvest period for the solid culture of the strain to produce spores is 7-10 days.

[0030]

strain

culture medium

sporulation start time

Spore production (spore

sub / ml)

Ratio ( / PDA)

Nr04-1

Nr04-2

Nr04-3

Nr04-4

Nr030808

...

Embodiment 3

[0035] Pest Control Test

[0036] 1. Symptoms of insect infection

[0037]After the larvae of Spodoptera litura and Spodoptera litura are infected by Nomura spp., they generally lose their appetite in about 3 days, start to act slowly, and start to die after 4 to 5 days. The peak death period is on the 6th to 8th day after treatment. Body color did not change significantly. After the dead insects were cultured at 26°C for 1 day, a small amount of white hyphae grew out of the insects. After 2 to 3 days, the mycelium gradually became dense and covered the insects, and the insects became stiff. Then the worm body was covered with green spore powder, and after 4 days, a dense light green pile was formed.

[0038] 2. Infection route

[0039] Infection of beet armyworm with the same concentration of spore suspension, regardless of body surface contact or oral infection, has higher activity on beet armyworm larvae. Three days after inoculation, the mortality rate of larvae expose...

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PUM

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Abstract

A fungal insecticide for preventing and eliminating the lepidopterous pests is prepared from Nomurace rilevi through separating in the field purifying in lavatory to obtain strain Nr04-3, culturing, and liquid-solid fermenting to obtain its spore powder.

Description

technical field [0001] The invention relates to a fungal insecticide, in particular to the production and application of an entomopathogenic fungus Nomura reinerii. Background technique [0002] At present, the quality and safety of agricultural products has become the focus of social attention, which has attracted the attention of governments at all levels in our country. Promoting the application of biological pesticides to replace chemical pesticides, especially highly toxic and high-residue chemical pesticides, can not only reduce pesticide residues and improve the quality of agricultural products, but also is an effective way to protect the environment and promote sustainable agricultural development. [0003] There are about 30 kinds of biological pesticides used in the world, among which the bacterial insecticide Bacillus thuringiensis (Bt) is the most researched, the largest yield, the widest range of applications, and the most representative biopesticide. At presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P7/00
Inventor 徐健刘琴殷向东
Owner JIANGSU LIXIAHE REGION AGRI RES INST
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