Use of POLY-GLU,TYR and T cells treated therewith for neuroprotective therapy
A technology of nerve regeneration and effect, applied in the fields of nervous system diseases, nervous system antigen components, neuromuscular system diseases, etc., can solve the problems of insufficient and no neuroprotective effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0108] Example 1. Physiological T cell repertoire of contused animals
[0109] SPD rats were anesthetized and their spinal cords were exposed at T8 level by laminectomy. One hour after induction of anesthesia, a 10 g rod was dropped from a height of 50 mm onto the laminectomized spinal cord using the NYU impactor (Basso et al., 1995 and 1996).
[0110] Eight to ten days after spinal cord contusion, rats were sacrificed and their spleens were excised and squeezed through a fine wire mesh. The washed cells (2×10 6 / ml) were cultured in triplicate in each well of a flat-bottomed microtiter plate with 0.2 ml of proliferation medium containing DMEM supplemented with L-glutamine (2 mM), 2-mercaptoethanol (5× 10 -5 M), sodium pyruvate (1 m M), penicillin (100 IU / ml), streptomycin (100 μg / ml), non-essential amino acids, and 1% (vol / vol) autologous macrophage with antigen (15 μg / ml) or Con A (1.25 μg / ml) Rat serum and irradiated thymocytes (2000 rad, 2×10 6 cells / ml). By measur...
Embodiment 2
[0111] Example 2. Protection of Optic Nerve Fibers from Glutamate Toxicity
[0112] To find out whether poly-Glu,Tyr could affect more general neuroprotection from harmful environmental conditions caused by glutamate-induced toxicity, the following experiments were performed.
[0113] Injection of the excitatory neurotransmitter glutamate into the vitreous of C57B1 / 6J mouse eyes caused dose-dependent death of optic neuron cell bodies. Previous studies have shown that the onset of RGC death is delayed (by more than 24 hours after glutamate injection) and resembles apoptosis.
[0114] In this experiment, 8-week-old male C57B1 / 6J mice were subcutaneously immunized with 100 μg of poly-Glu, Tyr emulsified in CFA 7 days before glutamate injection. A group of mice was simultaneously immunized with PBS emulsified in CFA to eliminate non-specific effects of immunization, while a group of unimmunized mice was used as a control. All three groups of mice received injections of glutamate...
Embodiment 3
[0117] Example 3. Neuroprotection of Spinal Cord Injury
[0118] Acute incomplete spinal cord injury at the low thoracic level causes an immediate loss of hindlimb voluntary movement that recovers spontaneously and stabilizes at defective locomotion within the first 12 days after injury. The amount of motor recovery was the sum of the positive effects of recovery from spinal shock and the negative effects of longitudinal and ventral spread of the injury. A treatment regimen aimed at reducing the spread of injury through neuroprotection will lead to a recovery in the right direction in terms of hindlimb voluntary movement.
[0119] In the following experiment, the effect of active or passive immunization with poly-Glu, Tyr on hindlimb voluntary movement after spinal cord contusion was tested.
[0120] 3.1 Active immunization with poly-Glu, Tyr: Effect of poly-Glu, Tyr / CFA immunization on recovery from spinal cord contusion in rats
[0121] Using the NYU impact device, a 10g r...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More