Method for preparing megakaryocytic preparation by amplifying macronucleus ancestral cell and mature megacaryocyte and use

A technology for mature megakaryocytes and megakaryotic progenitor cells, applied in biochemical equipment and methods, extracellular fluid diseases, medical preparations containing active ingredients, etc., can solve problems such as high price and poor clinical applicability
CN1554748AInactive Publication Date: 2004-12-15INST OF HEMATOLOGY & BLOOD HOSPITAL CHINESE ACAD OF MEDICAL SCI
0 Cites 5 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF HEMATOLOGY & BLOOD HOSPITAL CHINESE ACAD OF MEDICAL SCI
Publication Date
2004-12-15
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to mainly one system of efficient culturing and directive inducing stem cell differentiation for culturing and proliferating megakaryocyte. The system has umbilical cord blood and marrow stem cell as seed cell, and the serum-free culture medium comprising TPO, IL-11 and heparin as the main component for megakaryocyte proliferation. The megakaryocyte preparation prepared based on the present invention contains great amount of macronucleus ancestral cells and mature megakaryocyte as well as CD34+ cells, and has the functions of treating thrombocytopenia and improving the blood-forming function after stem cell transplantation. The present invention provides novel efficient cell treating preparation for various thrombocytopenia and hemocyitopenia.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The present invention relates to a method for preparing a megakaryocyte preparation, especially using stem cells derived from hematopoietic tissue (umbilical cord blood and bone marrow) as seeds, and combining thrombopoietin (TPO), interleukin 11 (IL-11) and heparin as megakaryocyte expansion Amplification system of stimulating factor, method and application of preparing megakaryocyte preparation by expanding megakaryotic progenitor cells and mature megakaryocytes. Background technique

[0002] Platelets are blood cells necessary to maintain normal blood clotting and the integrity of blood vessel walls in the body. Abnormal numbers of platelets can lead to blood and thrombotic diseases. The incidence of thrombocytopenia is relatively high, and patients need regular treatment due to repeated bleeding, which seriously endangers their physical and mental health, and brings a heavy economic burden to patients, and even life-threatening bleeding in severe c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More