Method for making frozen spermatozoon of yak
A production method and a technology of freezing semen, which are applied in medical science, veterinary instruments, animal delivery, etc., can solve the problems of high cost of live cattle introduction, loss of good genes, and short service life, and overcome the problems of ecology and feeding management. Difficulty, prevention of crossbreeding, and high fertilization rate
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Embodiment 1
[0067] Three breeding bulls were selected from the ranch in Hongba Township, Jiulong County, and their parentage and descendants were investigated, and their body size and appearance were measured. See Table 1 for details.
[0068] serial number
[0069] Unit: cm, kg
[0070] After more than 30 days of hard work, the technicians have achieved the purpose of semen collection. All the technical indicators of the semen have met the requirements, and the vitality after thawing is between 0.3-0.5. See Table 2 for details. The deformity rate was calculated by staining the semen smear with 0.5% Congo red alcohol solution.
[0071] serial number
[0072] The semen collected above has been made into frozen semen granules according to the method of the present invention, as shown in Table 3, wherein the diluent adopts glucose-yolk liquid.
[0073] make up
[0074] 2
[0075] The results showed that all technical indicators met the requirements,...
Embodiment 2
[0077] Three breeding bulls were selected from the pasture in Tanggu Township, Jiulong County. Semen was collected 15 times, 10 times successfully, and 8 times met the requirements for making frozen semen. A total of 689 granular frozen semen were produced. See Table 4 for details. The deformity rate was calculated by staining the semen smear with 0.5% gentian violet alcohol solution. The yak number 1 is 3.5 years old, the yak number 2 is 8 years old, and the yak number 3 is 5 years old.
[0078] make up
No
ejaculation volume
(ml)
color
the smell
PH value
Density (billion
/ ml)
Deformation rate%
Remark
once
secondary
1
2.0
light red
fishy smell
bloody urine
2
2.5
milky white
none
0.80
There is dirt
3
3.0
milky white
none
6.5
15.3
0.75
1...
Embodiment 3
[0083] Selected 3 breeding bulls from Yajiageng Ranch in Yulin Township, Kangding County, collected semen 25 times in total, 20 times successfully, and 17 times met the requirements for making frozen semen. A total of 1027 granular frozen semen were produced, 42 of which did not meet the requirements According to the breeding requirements, 985 frozen semen that meet the breeding requirements were obtained.
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