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Method for producing enzyme modified soybean lecithin

A technology of soybean lecithin and production method, applied in the direction of fermentation, etc., to achieve the effects of mild reaction conditions, strong functionality, and safe and reliable products

Inactive Publication Date: 2005-06-29
SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional chemical modification has been limited in use due to the introduction of other chemical substances.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Weigh 50kg of concentrated phospholipids into the reactor, adjust the temperature to 60°C, pH 7, add 1.25 million IU of phospholipase A 2 and 17g CaCl 2 Thoroughly stir evenly, and react for 8 hours under constant temperature condition of 60°C. Add 1 million IU of compound protease, control the pH value to 8, and react for 4 hours. The reactant is put into a centrifuge, centrifuged at 5000 rpm for 10 minutes to remove grease, and the residue is the modified phospholipid product.

Embodiment 2

[0018] Weigh 50kg of concentrated phospholipids into the reactor, adjust the temperature to 40°C, pH 6, add 1 million IU of phospholipase A 2 and 17g CaCl 2 Thoroughly stir evenly, and react for 8 hours under constant temperature condition of 45°C. Add 800,000 IU of compound protease, control the pH value to 8, and react for 3 hours. The reactant was put into a centrifuge, and centrifuged at 4000 rpm for 15 minutes to remove grease, and the residue was the modified phospholipid product.

Embodiment 3

[0020] Weigh 50kg of concentrated phospholipids into the reaction kettle, adjust the temperature to 50°C, pH 8, add 2 million IU of phospholipase A 2 and 17g CaCl 2 Thoroughly stir evenly, and react for 8 hours under constant temperature condition of 55°C. Add 1.2 million IU of complex protease, control the pH value to 8, and react for 5 hours. The reactant was put into a centrifuge, and centrifuged at 6000rpm for 20 minutes to remove grease, and the residue was the modified phospholipid product.

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Abstract

The invention provides a production method of enzyme-modified soybean lecithin, adding phospholipase A2 at 2000IU / 100g-4000IU / 100g concentrated phospholipid, adding CaCl2 at 0.003-0.005mol / L, constant temperature at 40°C-60°C, and reacting 6 ~8 hours; add compound protease, dosage 10000IU / g~20000IU / g, control pH value 6~8, react for 3~5 hours; centrifuge for 10~20 minutes, remove grease. The method of the present invention modifies soybean phospholipids by phospholipase A2, which not only improves its HLB value and enhances the processing characteristics such as water solubility of phospholipids, but also does not introduce chemical groups, and is safe, reliable, unlimited, widely used, and effective Significant advantages.

Description

【Technical field】 [0001] The invention relates to a production method of soybean lecithin, in particular to a production method of enzyme-modified soybean lecithin. 【Background technique】 [0002] Phospholipids are the main complex lipids in nature. Almost all organisms contain phospholipids, mainly in the brain, heart, liver, blood, milk, plant seeds and microorganisms of animals. The highest content is egg yolk and soybean. The main phospholipid products on the market are derived from soybeans. In the oil removal process, it is separated with the hydration oil, and then produced through concentration, refining and other processes. [0003] Phospholipids contain not only lipophilic fatty acids, but also hydrophilic phosphate groups. They are natural amphoteric surfactants, so they are widely used in various fields such as food, feed, light industry, chemical industry, and medicine. However, due to poor fluidity and poor water solubility of phospholipids, that is, its hyd...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P9/00
Inventor 江连洲孙树坤朱秀清梁歧
Owner SOYBEAN TECH DEV RES CENT HEILONGJIANG PROV
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