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Kit for testing trisome through method of molecular biology

A molecular biology and detection kit technology, applied in the field of mutation and genetic engineering, can solve problems such as the large psychological burden of pregnant women and their families, and achieve the effects of short detection time, alleviation of psychological pressure and mental burden, and simple use methods.

Inactive Publication Date: 2005-11-16
HONGHUI TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the cells need to be cultured, the entire testing process will take at least one month, and pregnant women and their families will bear a considerable psychological burden during this process

Method used

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Embodiment Construction

[0020] The present invention will be described in further detail below, and this technology is still clear to those skilled in the art.

[0021] The kit detects chromosomal diseases with aberrations in the number of chromosomes 21, 13, and 18 as follows:

[0022] (1) Prepare the reaction solution: the components of the reaction solution: include 1.5-3mM Mg 2+ , 100-300uM / ldNTP, 10-30pmol of STR primer and P.B.

[0023] Reaction Tube Number Chromosome Mapping

[0024] (3) Take out the test tube storing the reaction solution from the refrigerator at minus 20°C, and centrifuge at 5000 rpm for 10 seconds after the liquid is dissolved; after taking out the enzyme tube, centrifuge at 5000 rpm for 10 seconds;

[0025] (4) First use DNA Extractions to extract the DNA in the amniotic fluid of pregnant women.

[0026] (5) Prepare a mixed system: add enzyme and the DNA sample of amniotic fluid of pregnant women to be tested into the reaction solution tube; the amount of react...

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PUM

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Abstract

A reagent kit for molecular-biologic trisomy test of gravida features that its reactive liquid is composed of 2-valence Mg ions, dNTP, STR primer and Taq enzyme.

Description

[technical field] [0001] The invention relates to the fields of variation and genetic engineering, in particular to a molecular biology method trisomy detection kit. [Background technique] [0002] The traditional method of preventing the birth of children with congenital mental retardation requires pregnant women to undergo prenatal serum screening within 14 to 20 weeks of pregnancy. For pregnant women with high-risk prenatal serum screening, further prenatal diagnosis is required, that is, amniocentesis Obtain amniotic fluid cells, and conduct chromosome karyotype analysis by culturing the amniotic fluid cells to determine whether the fetus has chromosomal diseases. Since the cells need to be cultured, the entire testing process will take at least one month, and pregnant women and their families will bear a considerable psychological burden during this process. [0003] The present invention uses the STR gene scanning (genescan) analysis technology system to design and sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 邢晶晶
Owner HONGHUI TECH SHANGHAI
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