Method for breeding stock of rice temp-sensing sterile line and purifying origin temp

Abstract

A protospecies reproduction and origin temp purifying method for temp-sensitive sterile line of paddy rice is disclosed, which features that its selective breeding, protopecies reproduction and origin temp purifying can be conducted in the natural light temp condition. Its advantages are low cost and high safety.

Description

(1) Technical field: [0001] The invention belongs to the technical field of rice heterosis utilization, and in particular relates to the production of core seeds of rice temperature-sensitive male sterile lines, the propagation of original seeds and the genetic purification technology of starting point temperature. (two) background technology: [0002] The three-line hybrid rice not only has a complicated seed production procedure, but also has a relatively low chance of breeding an excellent combination due to the restriction of the strict recovery relationship. The discovery and application of the temperature-sensitive genic male sterile line has made the idea of ​​transforming hybrid rice from the "three-line method" to the "two-line method" a reality. The two-line hybrid rice does not require a maintainer line, the seed production procedure is simple, and the seed cost is reduced. Moreover, there is no recovery relationship between the parents of the two-line hybrid rice...

AI Technical Summary

Problems solved by technology

[0002] The three-line hybrid rice not only has a complicated seed production procedure, but also has a relatively low chance of breeding excellent combinations due to the strict recovery relationship.

However, this core seed production process still has the following technical problems: 1. The cost of core seeds produced is high; 2. Advanced equipment and facilities are required; 3. The "drift" of the starting point temperature cannot be completely prevented, and the symptoms are not cured.

[0005] After further research, some scholars believe that the essence of temperature-sensitive CMS starting point temperature rise is the change of genetic background, and the reason is that the CMS does not reach homozygous genetically.

Some scholars believe that the internal reason for the unstable expression of the fertility of the existing temperature-sensitive CMS is likely to be the impure genetic basis of the CMS at the starting temperature, that is, there are still a few genes that control the starting temperature. heterozygous state

It is further pointed out that although the production process of "core seed - original seed - original seed - improved seed" can guarantee the safety of seed production to a certain extent through the "core seed production process", it can only reduce the risk of high temperature sensitive strains. The proportion in the original population does not prevent the occurrence of "drift"

Moreover, the production process requires a human-controlled climate greenhouse, and the original species can only be used for one generation, and the cost is relatively high. It is difficult for grassroots scientific research units and enterprises that lack advanced equipment and facilities.

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