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Cow brain tissue sampling method

A technique of tissue sampling and bovine brain, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve time-consuming and labor-intensive problems

Inactive Publication Date: 2006-11-22
HUNAN CANWA BIOSCI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Obviously, such a sampling method is time-consuming and labor-intensive

Method used

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  • Cow brain tissue sampling method
  • Cow brain tissue sampling method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 5 hybrid cattle (Simmental and Charolais), aged 12-24 months, with a weight of 493-607kg. Saw the bovine skull from the midsagittal plane, measure the shortest distance from the bovine cranial cavity to the body surface, that is, the thinnest point. This point is located within 1-2 cm directly in front of the midpoint of the anterior edge line of the root of the angular process 1, and this point is selected as the needle insertion point 2. From 0°, 25±1° direction (see figure 2 Insert the needle in direction A and direction B), measure the depth to the cerebrum, cerebellum and brainstem, and take the average value and standard deviation of 5 cows. The data are shown in Table 1.

[0021]

Ear number

year

age

moon

body

Heavy

kg

needle

angle

Transcranial

length

cm

take brain group

Needle entry

length range

cm

Take th...

Embodiment 2

[0026] see figure 1 , 2 , within the range of 1 cm in front of the midpoint of the leading edge of the root of the angular process provided by the present invention, select this place as the needle insertion point. Use an electric drill and a drill bit with a diameter of 3.5 to drill a hole, insert the needle from the 0° direction (vertical) to a depth of 4.5cm, withdraw the needle core, suck 2g of bovine brain tissue with negative pressure, pull out the puncture needle, and put 2g of bovine brain tissue into the sample collection The bottle is sealed and sent for inspection. If the brainstem tissue is taken, the needle insertion depth in the 0° direction is 8 cm, withdraw the needle core, suck 2 g of the bovine brainstem tissue under negative pressure, pull out the puncture needle, put 2 g of the bovine brainstem tissue into the sample collection bottle and seal it. Submit for inspection.

Embodiment 3

[0028] see figure 1 , 2 , within the range of 2cm in front of the midpoint of the leading edge of the root of the angular process provided by the present invention, select this place as the needle insertion point. If the cerebellum tissue is taken, use an electric drill and a 5.5-diameter drill to drill a hole, insert the needle at a depth of 10 cm from the backward direction of 25°, withdraw the needle core, suck 2 g of bovine cerebellum tissue with negative pressure, pull out the puncture needle, and put 2 g of bovine cerebellum tissue into the Put it into the sample collection bottle, seal it, and send it for inspection. If the brain tissue is taken, the needle insertion depth in the 25° direction is 5 cm, withdraw the needle core, suck 2 g of bovine brain tissue with negative pressure, pull out the puncture needle, put 2 g of bovine brain tissue into a sample collection bottle, seal it, and send it for inspection. If the brainstem tissue is taken, the needle insertion de...

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Abstract

The present invention relates to brain tissue sampling method, and is especially ox brain tissue sampling method. The technological scheme of the present invention includes the following steps: 1. locating the sampling range 1-2 cm before the midpoint of ox horn root line; 2. drilling hole through the ox skull in the location; and 3. puncturing with a biopsy needle and sampling the required giant brain, little brain or brain stem tissues. The method of the present invention is simple, convenient and accurate.

Description

technical field [0001] The invention relates to a brain tissue sampling method, in particular to a bovine brain tissue sampling method. technical background [0002] The scientific name of mad cow disease is bovine spongiform encephalopathy, which is a progressive, highly lethal, and neurological disease of adult cattle. The clinical manifestations of sick cattle are hypersensitivity to vision, touch, and hearing, vegetative nerve regulation disorders, and ataxia. The typical pathological change is the degeneration of spongy vacuoles in specific areas of gray matter in the brainstem of sick cattle. Scientific investigations and studies have confirmed that the disease originated from scrapie in sheep, and can be transmitted to humans through contaminated food or biological products, causing people to be infected with Variant CJD and die. [0003] There is no effective treatment and simple early diagnosis method for mad cow disease, so it has become the most threatening anima...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61B10/00A61D1/00
Inventor 祝天经
Owner HUNAN CANWA BIOSCI DEV
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