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Ellipsometric biosensor comprising an amplification layer

An adsorption layer and layer system technology, applied in the field of biosensors, can solve problems such as limiting the sensitivity of ellipsometry detection

Inactive Publication Date: 2007-03-14
OC OERLIKON BALZERS AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Unfortunately, biological substances such as proteins or DNA have a refractive index close to that of glass or transparent plastic materials, limiting the sensitivity of ellipsometric detection when these organic substances are adsorbed to such substrates

Method used

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  • Ellipsometric biosensor comprising an amplification layer
  • Ellipsometric biosensor comprising an amplification layer

Examples

Experimental program
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Embodiment Construction

[0024] Since the optimal arrangement of the amplification layer system depends on the operating conditions of the biosensor, we will illustrate an example of a measurement configuration using a PCSA ellipsometer for measurements of binding dynamics. This device comprises a light source emitting a collimated beam passing through a variable polarization controller given by the combination of a first linear polarizer (P) and a compensator (C) in the form of a quarter-wave plate. The polarized beam is incident on the sample substrate (S) at a known oblique angle, is reflected at the detection surface of the sample substrate, and is analyzed by a second linear polarizer (A) (by means of a photodetector).

[0025] For such measurements, the detection surface must be brought into contact with a solution containing various molecules in order to adsorb or bind said molecules. Preferably, the above-mentioned detection surface is in a flow cell (on which surface a controlled flow of anal...

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Abstract

A method for analyzing organic adsorbent layers (7) on a substrate (4) comprises the steps of providing a substrate (4) whose surface has an index of refraction equal or close to the index of refraction of the organic adsorbent to be analyzed. On the surface of the substrate (4) there is applied a layer system (5, 6) with at least one layer (5) with index of refraction of the biological material and an organic adsorbent layer (7) on top of the layer system. When acting polarized light upon the substrate a change of polarization characteristics in reflection and / or transmission is detected.

Description

technical field [0001] The present invention relates to biosensors for detecting adsorption and binding processes of biologically related molecules at surfaces. Background technique [0002] Detection of binding reactions of biologically relevant molecules, such as antibody-antigen reactions, plays an important role in many biotechnology applications such as drug screening. To monitor such reactions, photometric detection is typically performed using a fluorescent tag attached to at least one of the various molecules involved. [0003] However, in addition to the extra effort required to label the molecule, it is also suspected that the presence of such labels alters the dynamics of the reaction. Thus, label-free detection schemes, in which no labels such as fluorescent labels, nanoparticles or radiolabels need to be attached to the molecule of interest, are gaining in importance. [0004] Several label-free detection schemes have been developed, which allow direct detecti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/21
CPCG01N2021/7773G01N21/211G01N21/75
Inventor A·艾恩M·维基J·埃德林格D·赫尼希
Owner OC OERLIKON BALZERS AG