Liposome-containing radiographic contrast medium and preparation method thereof

A technology for radiography and contrast agents, which is applied in the preparation of X-ray contrast agents, liposome delivery, and medical preparations with inactive ingredients, etc., and can solve problems such as failure to achieve results.

Inactive Publication Date: 2007-06-27
KONICA MINOLTA MEDICAL & GRAPHICS INC
View PDF12 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is further pointed out that because liposomes are almost always trapped in the reticuloendothelial tissue system after being i

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Liposome-containing radiographic contrast medium and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Determination of iodine in iodine compounds

[0115] The sample (liposome dispersion) was dialyzed with isotonic saline solution. After the dialysis, ethanol was added therein to destroy the liposomes, and then the amount of iodine compound encapsulated in the liposomes was measured by absorptiometry. The ratio of the above-mentioned amount of iodine compound to the total amount of iodine compound in the sample was used to represent the encapsulation efficiency (wt %).

[0116] Preparation of contrast agent

[0117] The mixture of 86mg dipalmitoylphosphatidylcholine (DPPC), 38.4mg cholesterol and 19.2mg PEG-phospholipids (SUNBRIGHT DSPE-020CN, the lipid modified by polyethylene glycol, by NIPPON OIL & FATS CO., LTD. production) into a stainless steel autoclave, heated to keep the temperature of the still at 60°C, and then, 13g of liquid carbon dioxide was added thereto. While stirring, make the pressure in the kettle by reducing the volume inside the autoclave by 50kg...

Embodiment 2

[0124] contrast agent

[0125] The preparation of samples 2-1 to 2-8 is the same as that of sample 1-1 in Example 1, except that the amount or type of contrast medium solution is different.

[0126] The iodine content of the sample thus prepared was measured, and the encapsulation efficiency (ie, the ratio of iopamidol encapsulated inside the liposome to the total amount of iodine compound) was determined by a spectrophotometer, as shown in Table 2.

[0127] Table 2

[0128] sample

Embodiment 3

[0130] Liposome vesicle size

[0131] Liposome vesicle size was determined by the following method. After the dispersion containing liposome vesicles encapsulated with iodine compound was frozen and fractured, carbon was evaporated and deposited on the fracture interface, and the deposited carbon was observed by electron microscope (freeze fracture TEM method).

[0132] Particle size was defined as the arithmetic mean of the sizes of the 20 liposomal particles observed.

[0133] Preparation of contrast agent

[0134] A mixture of 192 mg of dipalmitoylphosphatidylcholine (DPPC), 76.8 mg of cholesterol and 38.4 mg of PEG-phospholipid (SUNBRIGHT DSPE-020CN, polyethylene glycol-modified phospholipid, produced by NIPPON OIL & FATS CO., LTD. ) into a stainless steel autoclave, heated to keep the temperature of the still at 60°C, and then, 13g of liquid carbon dioxide was added thereto. While stirring, make the pressure in the kettle by reducing the volume inside the autoclave by ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Average sizeaaaaaaaaaa
Login to view more

Abstract

A liposome-containing X-ray radiopaque dye comprising a water soluble iodide compound contained in a water phase inside and outside lipid membranes. The liposomes are produced by mixing lipid membrane components for constituting lipid membranes with carbon dioxide of supercritical or subcritical condition in the presence of at least one compound having a hydroxyl or polyalkylene oxide group. The obtained X-ray radiopaque dye containing these liposomes does not contain any toxic solvents, such as chlorinated solvents, so that high safety can be ensured.

Description

technical field [0001] The invention relates to a radiographic contrast agent comprising liposomes and a preparation method thereof, in particular to a radiographic contrast agent comprising liposome vesicles. The calcium contrast agent is obtained by combining lipid membrane components with supercritical or subcritical carbon dioxide at least A mixture is formed in the presence of a compound containing a hydroxyl group or a polyoxyalkylene group, and the non-ionic iodine compound is efficiently and stably encapsulated in the liposome. Background of the invention [0002] The use of X-ray inspection and diagnosis is the core of today's medical imaging diagnosis. So-called hard tissues such as bones and teeth absorb X-rays efficiently, so high-contrast X-ray images can be obtained. Conversely, the differences in X-ray absorption among different soft tissues are relatively small, making it difficult to obtain high-contrast images. In such cases, contrast agents are often use...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K49/04A61K9/127A61K47/24A61K47/28A61K47/34A61K47/44A61K47/10
Inventor 上田荣一中岛彰久长池千秋元杭康之
Owner KONICA MINOLTA MEDICAL & GRAPHICS INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap