Cosmetic compositions containing substituted amine derivatives
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[0051] Compounds A through E were tested for their potential to suppress sebum expression, alone or in the presence of a retinoid.
[0052] Secondary cultures of human sebocytes obtained from an adult male were grown in 48-well tissue culture plates (Costar Corp.; Cambridge, MA) or 96- well tissue culture plates (Packard Co.; Meriden, CT) until confluent.
[0053] Sebocyte growth medium consisted of Clonetics Keratinocyte Basal Medium (KBM) supplemented with 14 .mu.g / ml bovine pituitary extract, 0.4 .mu.g / ml hydrocortisone, 5 .mu.g / ml insulin, 10 .mu.g / ml epidermal growth factor, 1.2 .times.10-10 M cholera toxin, 100 units / ml penicillin, and 100 .mu.g / ml streptomycin.
[0054] All cultures were incubated at 37.degree. C. in the presence of 7.5% CO.sub.2. Medium was changed three times per week.
[0055] On the day of experimentation, the growth medium was removed and the sebocytes washed three times with sterile Dulbecco's Modified Eagle Medium (DMEM; phenol red free). Fresh DMEM was added to e...
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