Method of treatment using electroporation mediated delivery of drugs and genes

a technology of electroporation and gene delivery, applied in the field of treatment of ailments, can solve the problems of difficult to properly locate electrodes and measure the distance between, and achieve the effect of convenient and effective positioning to genera

Inactive Publication Date: 2005-09-01
DEV S B +5
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008] Accordingly, it is a primary object of the present invention to provide an improved apparatus that can be conveniently and effectively positioned to generate predetermined electric fields in pre-selected tissue.

Problems solved by technology

When internal tumors are to be treated, it is not easy to properly locate electrodes and measure the distance between them.

Method used

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  • Method of treatment using electroporation mediated delivery of drugs and genes
  • Method of treatment using electroporation mediated delivery of drugs and genes
  • Method of treatment using electroporation mediated delivery of drugs and genes

Examples

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example 1

[0080] The tumor cell line Pant-3, a poorly differentiated adenocarcinoma cell line of the pancreas, was supplied by Anticancer, Inc., San Diego. For ECT experiments, tissue taken from the stock mice, where the tumor line was maintained, was thawed and cut into very small pieces about 1 mm each, and 8-10 pieces were surgically xenografted in a subcutaneous sac made in left flank of nude mice, and then closed with 6.0 surgical suture. After the average tumor size reached about 5 mm, mice with palpable tumors were divided randomly, 10 mice for control group (D+E−; D=Drug, E=Electric field) and 10 mice for ECT treatment, namely bleomycin injection followed by pulsing (D+E+) from a BTX Square Wave T820 Generator. The tumor dimensions were measured and the tumor volume calculated using the formula:

(II / 6)×a×b×c

where a, b, and c are, respectively, the length, width and thickness of the tumor. 0.5 units Bleomycin (Sigma Chemicals) was dissolved in 0.15 ml of 0.9% NaCl and was injected in...

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Abstract

A method for in vivo electrotherapy, or electroporation-mediated therapy, using a needle array apparatus is provided. Treatment of tumors with a combination of electroporation using the apparatus of the invention, and a chemotherapeutic agent, caused regression of tumors in vivo.

Description

REFERENCE TO RELATED APPLICATIONS [0001] The present application is a Continuation-in-Part of co-pending application Ser. No. 08 / 467,566, filed on Jun. 6, 1995, which is a Continuation-in-Part of co-pending application Ser. No. 08 / 042,039 filed on Apr. 1, 1993.BACKGROUND OF THE INVENTION [0002] The present invention relates to the treatment of ailments in humans and other mammals, and more particularly, to an improved method and apparatus for the application of controlled electric fields for in vivo delivery of genes and pharmaceutical compounds into live cells of a patient by electroporation. [0003] In the 1970's it was discovered that electric fields could be used to create pores in cells without causing permanent damage to them. This discovery made possible the insertion of large molecules into cell cytoplasm. It is known that genes and other molecules such as pharmacological compounds can be incorporated into live cells through a process known as electroporation. The genes or ot...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61M37/00A61N1/32A61N1/30
CPCA61N1/0502A61N1/327A61N1/325C12M35/02
Inventor DEV, S.B.HOFMANN, GUNTER A.GILBERT, RICHARD A.HAYAKAWA, YASUHIKOHELLER, RICHARDJAROSZESKI, MARK J.
Owner DEV S B
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