Food product & process for manufacturing same
a technology for food products and processing methods, applied in the field of packaging food products, can solve the problems of unacceptably loose faeces output, poor control performance, and inability to accurately measure the effect of faeces, and achieve the effect of easy breaking into pieces
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experiment 1
In vitro Experiment 1
[0127] An in-vitro fermentation system was set up in order to assess the active ingredients for their potential to reduce hydrogen sulphide and total gas production. Ingredients were weighed into glass serum bottles containing fermentation media. Some bottles were also prepared without active ingredients in order to act as controls. Bottles were sterilized and placed into an anaerobic cabinet. Samples of faeces were collected, slurried, and aliquots of this slurry added to the control bottles and the bottles containing active ingredients—the bottles were then sealed and incubated for 24 hours at 37 C.
[0128] Hydrogen sulphide concentration was determined by the assay as set out.
[0129] Table 1a shows the mean hydrogen sulphide levels that were present in fermentation broths following incubation with active ingredients. There were significant reductions in hydrogen sulphide with the individual active ingredients (p<0.05). Surprisingly, a synergistic effect of add...
experiment 2
In vitro Experiment 2
[0130] The experiment described above was repeated using binary mixtures of the actives.
[0131] Table 1b shows the mean hydrogen sulphide levels that were present in fermentation broths following incubation. The hydrogen sulphide levels for the control and the combination of all 3 actives are broadly similar to those determined in the previous example. The effect of the charcoal and zinc acetate appears, surprisingly, to be synergistic.
TABLE 1bMean hydrogen sulphide (ppm) following treatment with active ingredientsHydrogen Sulphide (ppm) / (g)Wet Weight Faeces (n = 3)TreatmentMeanSEMedia and Faeces Control39819.863 Actives90.96.78Charcoal and Yucca174.6344.07Charcoal and Zinc80.274.14Zinc and Yucca209.362.65
In-vivo Experiment
[0132] Details of the novel in-vivo method are given above under the heading “In-vivo Method”. In these experiments a treat containing all 3 active ingredients was compared with a control containing no actives, as shown in the recipes belo...
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