Composite Nanomaterials for Photocatalytic Hydrogen Production and Method of Their Use
a technology of photocatalytic hydrogen and nanomaterials, which is applied in the direction of electrolytic organic production, on/in inorganic carriers, instruments, etc., can solve the problems of limited expression, and continuous catalyst cycling requires ongoing addition of reactants
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example 1
Encapsulation of Hydrogenases in Polymer Gel
[0059]This example is focused specifically on the hydrogen production activity of H2ase:sol-gel materials. Hydrogenases are bi-directional enzymes which also catalyze the oxidation of hydrogen. H2ase:sol-gel pellets were assayed for hydrogen oxidation activity by placing the pellets in buffered (pH 8.0) solution under a head pressure of hydrogen. Electron flow was monitored by the reduction of methyl viologen. H2ase:sol-gel pellets with either the NiFe (Lamprobacter modestogalophilus (Lm) and Thiocapsa roseopersicina (Tr)) or Fe-only (Clostridium pasteurianum (CpI)) forms of H2ase retain approximately 60-70% of the hydrogen evolution specific activity observed in solution (Table 1) (Isolation of hydrogenases: (a) L. modestogalophilus: Zadvorny, O. A.; Zorin, N. A.; Gogotov, I. N.; Gorlenko, V. M. Biochemistry (Mosc) 2004, 69, 164-169 (b) T. roseopersicina: Sheiman, M. B.; Orlova, E. V.; Smirnova, E. A.; Hovmoller, S.; Zorin, N. A. J Bacter...
example 2
Synthesis of Pt Nanoparticles Encapsulated within the Hsp Cage
[0065]This example describes the synthesis of Pt nanoparticles encapsulated within the Hsp cage. The self-assembled cage-like architecture of the small heat shock protein (Hsp) from Methanococcus jannaschii has been used to encapsulate metal clusters with a defined spatial arrangement (Flenniken et al. 2003). Hsp assembles from 24 subunits into a 12 nM cage defining a 6.5 nm interior cavity with pores through the cage architecture, by which molecules can shuttle between the inside and outside environment (FIG. 5) (Kim et al. 1998).
[0066]Briefly outlined, purified Hsp was incubated with PtCl42− at 65-C for 15 min. The protein cage was incubated with either 150, 250, or 1000 Pt per protein cage. Subsequent reduction with dimethylamine borane complex ((CH3)2NBH3) resulted in the formation of a brown solution. Characterization of the reaction product by size exclusion chromatography revealed retention volumes identical with u...
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