Soybean mutant strain and soybean oil therefrom

a technology of soybean oil and mutant strain, which is applied in the field of soybean mutant strain and soybean oil therefrom, can solve the problems of inability to avoid the limitation of its use for each plant species, and achieve the effects of improving quality, and reducing the content of polyunsaturated fatty acids

Inactive Publication Date: 2009-03-12
ANAI TOYOAKI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The mutant soybean strain effectively decreases the levels of linolic and linolenic acids, leading to the production of soybean oil with improved stability and reduced oxidation, thereby enhancing its storage and usage properties.

Problems solved by technology

The composition of the fatty acids is specific to plant species from which they are derived, and therefore, it is impossible to avoid limitation of its use for each plant species.

Method used

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  • Soybean mutant strain and soybean oil therefrom
  • Soybean mutant strain and soybean oil therefrom
  • Soybean mutant strain and soybean oil therefrom

Examples

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Effect test

examples

(1) Extraction of Genomic DNA

[0029]Frozen leaves were ground in a mortar, then, to 1 g of this powder was added 3 ml of 2% CTAB buffer [10 mM Tris-HCl (pH 8.0), 1.4 M NaCl, 20 mM EDTA, 2% CTAB], and the mixture was stirred gently. This was incubated on a water bath of 65° C. for 30 minutes and the same volume of chloroform was added and stirred gently, then, centrifugal separation was conducted at 4° C. under 8000 rpm for 10 minutes to remove proteins, and again, proteins in the aqueous layer were removed by extraction with chloroform. This aqueous layer was transferred to another centrifugal tube, and 1% CTAB precipitation buffer [50 mM Tris-HCl (pH 8.0), 1% CTAB] was added so that the total amount was 12 ml and they were mixed gently, then, centrifugal separation was conducted at 4° C. under 8000 rpm for 10 minutes to precipitate a CTASB-DNA complex. The supernatant was discarded, then, the precipitate was dissolved in 4 ml of 1M NaCl for removal of CTAB, and 4 ml of isopropanol w...

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Abstract

A soybean oil of a decreased content of polyunsaturated fatty acids is produced by hybridizing, selecting and raising a soybean mutant KK21 characterized by a gene having a sequence of base given by the sequence No. 1 of the sequence listing, a soybean mutant M23 characterized by deletion of a gene having a sequence of base given by the sequence No. 2 of the sequence listing, a soybean mutant M24 characterized by a gene having a sequence of base given by the sequence No. 3 of the sequence listing, a soybean mutant M5 characterized by a gene having a sequence of base given by the sequence No. 4 of the sequence listing, a soybean mutant J18 characterized by deletion of a gene having a sequence of base given by the sequence No. 5 of the sequence listing, and one or two kinds or more of these soybean mutants.

Description

[0001]This application is a divisional of application Ser. No. 10 / 419,865, filed Apr. 22, 2003, now allowed. The teachings of the above-referenced application is hereby incorporated by reference.BACKGROUND OF THE INVENTION[0002]The present invention relates to a novel mutant strain obtained by irradiation with X-rays using a soybean strain Bay as a new strain, and a soybean oil of a decreased content of polyunsaturated fatty acids obtained therefrom.[0003]In general, a triacylglycerol which is a main component of fats and oils present in plants, particularly their seeds, is a compound obtained by bonding three fatty acid molecules to one glycerol molecule, and depending on the kind of the bonded fatty acid, physical and chemical properties and nutritive values are different. The composition of the fatty acids is specific to plant species from which they are derived, and therefore, it is impossible to avoid limitation of its use for each plant species.[0004]On the other hand, for sup...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): C12Q1/68A01H5/00C07C57/00C12N9/02C12N15/01C12N15/82
CPCC12N15/8247C12N9/0083
InventorANAI, TOYOAKITAKAGI, YUTAKA
OwnerANAI TOYOAKI