CoQ10 (Ubiquinone, Ubiquinol), Vitamin A (Retinoid Acid, Retinol), Vitamin E (Tocotrienol, Tocopherol) and Methods of Use
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example 1
[0088]CoQ10 encapsulated supplements with various mixtures of vitamin E (tocopherol-free tocotrienols, tocopherol-tocotrienol mixtures, tocotrienol-free tocopherols, esterified vitamin E) were analyzed for ubiquinol and ubiquinone simultaneously by high performance liquid chromatography (HPLC) using a modified method of an existing collaborative study (Lunetta and Roman, 2008). Samples were prepped by expelling contents from softgels / capsules and transferring them quantitatively to a volumetric flask. Softgels were then rinsed exhaustively using ethanol, and rinses were transferred to the flasks. Sonication was used as needed to completely clean out the gels. Samples were standardized to volume with ethanol, then the flasks were stoppered and mixed thoroughly. Samples were diluted and immediately injected (20 uL injection volume) onto an HPLC manufactured by Thermo Separation Products with in-line degasser, P4000 pump, AS3000 Autosamples, and scanning detector. The column used was a...
example 2
[0100]Conditions were set as in Example 1. All encapsulated supplements with esterified vitamin E (e.g., alpha-tocopheryl acetate, alpha-tocopheryl succinate) saw zero conversion of ubiquinone to ubiquinol. Only vitamin E alcohols are effective.
example 3
[0101]Conditions were set as in Example 1. Encapsulated supplements without tocopherol and / or tocotrienol saw zero conversion of ubiquinone to ubiquinol, as shown in FIG. 4. Furthermore, the amount of CoQ10 was diminished by 20% of the labeled value. Vitamin E protects from degradation of CoQ10, and without added vitamin E no ubiquinol was detected.
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