Immunochromatographic kit and method for detecting mycobacterium tuberculosis

Abstract

An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detecting Mycobacterium tuberculosis with high-sensitivity and specificity. According to the present invention, an immunochromatographic kit for detecting Mycobacterium tuberculosis is provided, the kit including: a label substance modified with a first antibody against lipoarabinomannan; a porous carrier having a reaction site holding a second antibody against lipoarabinomannan; a compound containing silver; and a reducing agent reducing silver ions, in which at least one of the first antibody or the second antibody is a monoclonal antibody.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation of PCT International Application No. PCT / JP2019 / 034112 filed on Aug. 30, 2019, which claims priority under 35 U.S.C § 119(a) to Japanese Patent Application No. 2018-163097 filed on Aug. 31, 2018. Each of the above application(s) is hereby expressly incorporated by reference, in its entirety, into the present application.REFERENCE TO SEQUENCE LISTING SUBMITTED VIA EFS-WEB[0002]This application includes an electronically submitted sequence listing in .txt format. The .txt file contains a sequence listing entitled “2021-04-19_2870-0780PUS1_ST25.txt” created on Apr. 19, 2021 and is 13,087 bytes in size. The sequence listing contained in this .txt file is part of the specification and is hereby incorporated by reference herein in its entirety.BACKGROUND OF THE INVENTION1. Field of the Invention[0003]The present invention relates to an immunochromatographic kit for detecting Mycobacterium tuberculosis, the kit...

AI Technical Summary

Benefits of technology

[0008]An object of the present invention is to provide an immunochromatographic kit and a method, which are capable of detecting Mycobacterium tuberculosis with high-sensitivity and specificity.

[0009]As a result of intensive studies to achieve the above-mentioned object, the inventors of the present invention have found that it is possible to detect Mycobacterium tuberculosis with high-sensitivity and specificity by incorporating a compound containing silver, and a reducing agent capable of reducing silver ions, and by allowing at least one of a first antibody or a second antibody to be a monoclonal antibody, in an immunochromatographic kit for detecting Mycobacterium tuberculosis, the kit including a label substance modified with the first antibody against lipoarabinomannan, and a porous carrier having a reaction site holding the second antibody against lipoarabinomannan. Therefore, the inventors of the present invention have completed the present invention.

Problems solved by technology

Lack of diagnostic sensitivity, lack of specificity for Mycobacterium tuberculosis caused by cross-reaction with a nontuberculous mycobacterial group due to use of polyclonal antibodies, and the like have been pointed out in existing kits and methods for detecting lipoarabinomannan (LAM) and diagnosing tuberculosis, which is an infectious disease.

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