Production method of microbial antioxidant

Abstract

The invention provides a production method of microbial antioxidant, which is characterized by comprising the following specific steps: culturing Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in one culture medium; simultaneously inoculating the three strains into a broth culture medium for culturing, streaking a culture solution, and individually selecting the separated strains for pure culture; transferring the production strains into an eggplant-shaped flask filled with nutrient agar for culturing; inoculating the mixture of the Rhodopseudomonas palustris, the Saccharomyces cerevisiae and the Lactobacillus plantarum into a broth culture medium for strain propagation; and inoculating the strain subject to the propagation in a culture medium, and fermenting to obtain the microbial antioxidant. The invention can effectively eliminate active oxygen free radicals generated in vivo, protect the normal structure of cell membranes and ensure the normal functions of in vivo active molecules.

Description

technical field

[0001] The invention relates to a production method of a microbial antioxidant, which can promote the transformation and absorption of nutrients, improve the body's immunity and resistance, and is beneficial to the prevention and recovery of various diseases, belonging to the technical field of biological products. Background technique

[0002] Modern medicine has proved that the occurrence of many diseases in organisms is mostly induced by a series of free radicals on the covalent bonds of unsaturated fatty acids in the body. The latest research proves that oxygen free radicals can polymerize biological proteins, break nucleic acid main bonds, modify bases and destroy hydrogen bonds, thereby causing peroxidative denaturation, cross-linking or breaking of macromolecular substances in the body, causing damage to cell structure and function. Cause tissue damage and organ degenerative changes.

[0003] Excess free radicals in the body attack the lipids and prot...

Examples

Embodiment 1

[0028] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 25°C for 30 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.04%, Gynostemma pentaphyllum 0.1%, roxburghii 0.3%, wild honey 1%, kelp 2.5%, rice bran bean cake powder 4%, sterile water 92.06%;

[0029] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 35°C for 22 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture at 25°C for 30 hours in soup medium, and save as production strain;

[0030] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 35°C for 45 hours, and then store them in a refrigerator at 2°C ;

[0031] The fourth step: Inoculate th...

Embodiment 2

[0037] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 30°C for 28 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.02%, Gynostemma pentaphyllum 0.12%, roxburghii 0.4%, wild honey 1.3%, kelp 3.5%, rice bran bean cake powder 3%, sterile water 91.66%, pH value adjusted to 6.0, temperature 121 ℃, steam sterilization for 30 minutes;

[0038] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 40°C for 26 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture in soup medium at 30°C for 28 hours, and save it as the production strain;

[0039] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 40°C for 50 hou...

technical field

[0001] The invention relates to a production method of a microbial antioxidant, which can promote the transformation and absorption of nutrients, improve the body's immunity and resistance, and is beneficial to the prevention and recovery of various diseases, belonging to the technical field of biological products. Background technique

[0002] Modern medicine has proved that the occurrence of many diseases in organisms is mostly induced by a series of free radicals on the covalent bonds of unsaturated fatty acids in the body. The latest research proves that oxygen free radicals can polymerize biological proteins, break nucleic acid main bonds, modify bases and destroy hydrogen bonds, thereby causing peroxidative denaturation, cross-linking or breaking of macromolecular substances in the body, causing damage to cell structure and function. Cause tissue damage and organ degenerative changes.

[0003] Excess free radicals in the body attack the lipids and prot...

Examples

Embodiment 1

[0028] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 25°C for 30 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.04%, Gynostemma pentaphyllum 0.1%, roxburghii 0.3%, wild honey 1%, kelp 2.5%, rice bran bean cake powder 4%, sterile water 92.06%;

[0029] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 35°C for 22 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture at 25°C for 30 hours in soup medium, and save as production strain;

[0030] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 35°C for 45 hours, and then store them in a refrigerator at 2°C ;

[0031] The fourth step: Inoculate th...

Embodiment 2

[0037] Step 1: Cultivate Rhodopseudomonas palustris, Saccharomyces cerevisiae and Lactobacillus plantarum in the same medium at 30°C for 28 hours; the medium is composed of the following raw materials in weight percentage: Ganoderma lucidum spore powder 0.02%, Gynostemma pentaphyllum 0.12%, roxburghii 0.4%, wild honey 1.3%, kelp 3.5%, rice bran bean cake powder 3%, sterile water 91.66%, pH value adjusted to 6.0, temperature 121 ℃, steam sterilization for 30 minutes;

[0038] Step 2: Inoculate the three bacterial strains obtained in the first step into the broth culture medium at the same time, culture at 40°C for 26 hours, take the culture solution for streak separation, and separate the isolated strains in the meat Carry out pure seed culture in soup medium at 30°C for 28 hours, and save it as the production strain;

[0039] Step 3: Transfer the production strains obtained in the second step to eggplant bottles filled with nutrient agar medium, culture them at 40°C for 50 hou...