Genes whose expression is increased in response to stimulation by corticotropin-releasing hormone
An adrenal cortex, hormone-releasing technology, applied in the determination/inspection of microorganisms, peptide sources, drug combinations, etc., can solve problems such as unexplored main fields
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[0015] As used herein, the term "compound" or "agent" refers to a biological or chemical compound such as a simple or complex organic molecule, peptide, protein or oligonucleotide. A "test compound" as used herein refers to a "compound" or "agent" used in a method according to the invention to assess whether said compound modulates CRH signaling activity.
[0016] As used herein, "CRH signaling" refers to changes in the transcription of genes in a cell after CRH activates the corticotropin releasing hormone receptor in said cell. It thereby induces a CRH-specific gene expression profile. Changes in transcript levels can be assessed at the protein or gene-RNA level.
[0017] "CRH responsive activity" as used herein generally refers to changes in detectable cellular parameters resulting from exposure of cells to CRH. Detectable cellular parameters include changes in membrane potential, changes in the enzymatic activity of enzymes that regulate CRH signaling in said cell, chang...
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