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Preparation method of water solution with locust stems and leaves extraction for controlling cucumber powdery mildew

A technology of cucumber powdery mildew and extract, which is applied in the field of pesticides, can solve the problems of many impurities in antibacterial active ingredients and small biomass of broccoli, and achieve good preventive effect, high content, and simple extraction process

Inactive Publication Date: 2009-05-13
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the search, it was also found that the Chinese patent publication number CN1788572, the patent name "Preparation method for controlling powdery mildew of melons by using extracts of Radix Radix Sinensis", the patent mentioned that the ethanol extract of the whole plant of Radix Radix Radix L. Cucumber powdery mildew, but the biomass of Dijin in this method is not large, and there are more impurities in the antibacterial active ingredients

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The weight percentage content of the water agent for controlling cucumber powdery mildew: 0.2% of the stem and leaf extract of Robinia pseudoacacia, and 99.8% of distilled water.

[0023] Weigh 100 g of Robinia pseudoacacia stem and leaf powder (20 mesh particles), soak and extract twice with 500 ml of 60% ethanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 9.0 g of dry matter; use ultrasonic waves to suspend the dry matter with 100 ml of water , extracted 4 times with an equal volume of n-butanol, concentrated the aqueous phase extract to obtain 2.5 g of dry matter; dissolved the dry matter with 50 ml of distilled water, adsorbed it with 1000 g of macroporous adsorption resin for 24 hours, desorbed it with 1500 ml of distilled water, collected the lotion and concentrated it under reduced pressure After drying, 2.2 g of the extract was obtained, which was formulated into an aqueous solution with a conc...

Embodiment 2

[0025] The weight percent content of the water agent for controlling cucumber powdery mildew: 2% of Robinia pseudoacacia stem and leaf extract, and 99% of distilled water.

[0026] Weigh 100g of tomato stem and leaf powder (20 mesh particles), soak and extract 3 times with 1000ml of 80% ethanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 8.5g of dry matter; use ultrasonic waves to suspend the dry matter with 100ml of water , extracted 5 times with an equal volume of n-butanol, concentrated the aqueous phase extract to obtain 2.2 g of dry matter; dissolved the dry matter with 40 ml of distilled water, adsorbed with 750 g of macroporous adsorption resin for 24 hours, desorbed with 1250 ml of distilled water, collected the lotion and concentrated it under reduced pressure After drying, 2.0 g of the extract is obtained, and it is prepared into an aqueous solution with a concentration of 2%. An indoor pot test is ...

Embodiment 3

[0028] The weight percent content of the aqueous agent for controlling cucumber powdery mildew: 4% of the stem and leaf extract of Robinia pseudoacacia, and 96% of distilled water.

[0029] Weigh 100g of tomato stem and leaf powder (20 mesh particles), immerse and extract 3 times with 2000ml of absolute ethanol at room temperature, each time for 24 hours, concentrate under reduced pressure and recover the solvent to obtain 7.2g of dry matter; use ultrasonic waves to suspend the dry matter with 100ml of water , extracted 5 times with an equal volume of n-butanol, concentrated the aqueous phase extract to obtain 2.0 g of dry matter; dissolved the dry matter with 30 ml of distilled water, adsorbed it with 500 g of macroporous adsorption resin for 24 hours, desorbed it with 1000 ml of distilled water, collected the lotion and concentrated it under reduced pressure After drying, 1.7 g of the extract is obtained, and it is prepared into an aqueous solution with a concentration of 4%....

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Abstract

The present invention relates to a method capable of utilizing yellow locust stem and leaf extract to prepare aqueous preparation for effectively controlling powdery mildew of cucumber. Said aqueous preparation composition includes (by wt%) 0.2-4% of yellow locust stem and leaf extract, and the rest is water. Besides, said invention also provides the preparation method of said aqueous preparation and its concrete operation steps.

Description

technical field [0001] The invention relates to a preparation method in the technical field of pesticides, in particular to a preparation method of an aqueous solution for controlling cucumber powdery mildew with the stem and leaf extract of Robinia pseudoacacia. Background technique [0002] Cucumber powdery mildew, caused by Sphaerotheca fuliginea (Schlecht) Poll., is a short incubation period and highly prevalent leaf disease, which can generally reduce cucumber yield by 5-20%, and is an important factor limiting the high and stable yield of cucumber. At present, chemical pesticides are mainly used in production to control the disease, but powdery mildew has been found to be effective against seven types of fungicides (benzimidazoles, EBIs, organophosphorus, hydroxypyrimidines, triazoles, methoxyacrylates and Phenoxyquinolines) developed drug resistance almost at the same time, and the speed of resistance development was astonishing; at the same time, due to the long-term...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/00A01P3/00A01N65/20
Inventor 代光辉张志远诸葛媛媛李艳波
Owner SHANGHAI JIAOTONG UNIV
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