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Biomarking monitoring method of environment pollution caused by As

A technology for biomarkers and arsenic contamination, which is applied in soil testing, measuring devices, material inspection products, etc., can solve the problem that the same or similar literature reports on biomarker monitoring methods have not been found, and is suitable for long-term monitoring and has high sensitivity. Effect

Inactive Publication Date: 2009-10-28
SHANGHAI JIAOTONG UNIV
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Problems solved by technology

[0004] After checking the novelty of the prior art, no literature reports identical or similar to the biomarker monitoring method of arsenic pollution, the subject of the present invention, have been found

Method used

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  • Biomarking monitoring method of environment pollution caused by As

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Embodiment Construction

[0015] Such as figure 1 As shown, the implementation steps of the embodiment of the present invention are as follows:

[0016] 1. First understand the background information of the monitoring site, determine the source of pollution and the liquid discharge or manure fertilization of the farm. Then determine the specific method of sampling.

[0017] 2. First collect the soil at the sampling point and put it into a container; then put the collected earthworms into the container and bring them back to the laboratory.

[0018] 3. Take out the earthworms in the container, put them on wet filter paper, and place them at room temperature for 1 to 2 days to discharge the intestinal contents; take the earthworms after bowel cleansing, absorb the water with filter paper, and place them in a glass homogenizer , poured into the earthworm homogenate buffer pre-cooled in a 4°C refrigerator, and homogenized; the homogenate was transferred into a 1.5ml centrifuge tube, and centrifuged for 3...

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Abstract

A biomarker monitoring method for environmental arsenic pollution in the field of environmental protection technology. The present invention first designs a soil sampling plan according to the site to be monitored; then collects earthworms at each sampling point and puts them into a container containing the soil of the sampling site; takes the collected earthworms, first performs intestinal cleaning, then homogenizes, and centrifuges , the supernatant is the required extract containing P450; measure the activity of 7-methoxyl-3-isophenoxazolone-O-debenzylase in a fluorescence spectrophotometer; calculate according to its activity The total arsenic content in the soil can be used to monitor the degree of arsenic pollution in the soil. The invention can not only understand the degree of soil arsenic pollution, but also understand the biochemical effects of arsenic pollution and the response of organisms to arsenic pollution. It has more practical value than direct determination of soil arsenic content. And high sensitivity, suitable for long-term monitoring.

Description

technical field [0001] The present invention relates to a method of environmental protection technology, specifically a biomarker monitoring method for environmental arsenic pollution, that is, using cytochrome P450 as a biomarker to monitor the surrounding environment of pig farms and the soil environment where pig manure has been used methods of arsenic contamination. Background technique [0002] Arsenic is an essential trace element in animals and has many important functions. In recent years, arsenic preparations have become feed additive drugs widely used in countries all over the world, which has promoted the development of animal husbandry and achieved good social and economic benefits. But at the same time, a large amount of livestock and poultry excrement containing arsenic enters the environment, causing pollution to the environment. Its pollution objects are mainly the surrounding environment of the farm and the soil environment where pig manure has been used. ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/24G01N21/17G01N21/64
Inventor 李银生邱江平陈晓斌曾振灵陈杖榴
Owner SHANGHAI JIAOTONG UNIV
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