Method for screening super accumulative plant in flower plant
A super-accumulative and plant-based technology, applied in the field of phytoremediation, can solve the problems of limited species of super-accumulative plants, and achieve the effect of not causing secondary pollution, strong operability, and beautifying the environment
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Embodiment 1
[0023] Embodiment 1: to the pot plant concentration gradient experiment of Mirabilis mirabilis
[0024] Cd is a non-essential element for organisms that is toxic to plant growth and has "three effects" on the body. It is a representative pollutant in Liaoning and needs to be treated urgently.
[0025] The potting experiment site is in the net room of Shenyang Institute of Applied Ecology, Chinese Academy of Sciences. The site is in the center of Shenyang. There are no pollution sources around the experimental site, and it is an area not polluted by heavy metals. The total annual radiation dose is 520~544KJ / cm 2 , frost-free period 127 ~ 164d. The potting test soil was collected from the surface soil in the pollution-free area of the Shenyang Ecological Station of the Chinese Academy of Sciences, and the soil type was meadow brown soil.
[0026] The herbaceous flowers and plants were planted in potted soil with high concentration of heavy metals; the flowers and plants with...
Embodiment 2
[0034] Embodiment 2: to the hydroponic experiment of Mirabilis
[0035] Cd and Pb are the main heavy metal pollutants in the environment, and they often exist in nature. Therefore, the herbaceous flower plants are planted in the soil with high concentration of heavy metal pollution in the form of potted plants, and the flower plants with strong tolerance and accumulation are screened out. ; Then adopt the method of hydroponic experiment, cultivate Mirabilis seedlings in the environment of Cd-Pb compound pollution.
[0036] Robust and uniform-looking one-month-old seedlings were picked and their roots were submerged in 0.1% KMnO 4 The solution is 10 minutes, which can sterilize and promote plant rooting, and then rinse off KMnO with distilled water 4 Afterwards, it was cultured in a 300mL Erlenmeyer flask. In the pre-cultivation stage, the Erlenmeyer flask only contained nutrient solution. In order to avoid the interference between ions, only basic nutrients were selected in t...
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