Total jasminoidin extract and its prepn process

A technology for extracting glucosides and geniposide, which is applied in the field of total glucoside extract of jasmine and its preparation, can solve the problems of unclear material basis, many residual weak polar impurities, different composition and mutual ratio, etc. The effect of reducing the presence of impurities

Inactive Publication Date: 2007-11-07
PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The other is the gardenia iridoid glycoside extract, which measures the total amount of geniposide or gardenia iridoid glycosides, and the other main active ingredient of gardenia genipin-1-β- The content of D-gentiobioside has not been determined, resulting in inhomogeneous quality of gardenia extract, its essence is still a mixture, the material basis is not clear, its composition and mutual ratio vary by method, and it is not universal and widely used applicability
[0004] In the prior art, the extraction and purification methods of the total glycosides of gardenia mostly adopt the method of macroporous resin: (1) directly elute with high-concentration ethanol after water elution, this method not only cannot remove the slightly stronger geniposide The iridoid glycoside components of the geniposide, and a lot of weak polar impurities remain [Lv Maoping, Research on the separation effect of geniposide by macroporous resin, Chinese herbal medicine, 2002, 33 (9), 794; Yao Ping, Macroporous adsorption Resin purification of total iridoid glycosides and geniposide in Gardenia, Chinese Herbal Medicine, 2006, 37(1), 57; Chen Gang et al., Research on the purification of Gardenia extract for injection by macroporous adsorption resin, Chinese Journal of Traditional Chinese Medicine, 2005, 30(3), 188; Jin Rixian et al., Study on the process of enriching ge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0073] Extraction process:

[0074] Extract gardenia jasmine with 10% ethanol solution to obtain an ethanol extract, concentrate until it has no alcohol smell; adjust the pH value of the concentrate to 5.0; separate the solution by HPD-100 chromatography;

[0075] First remove impurities with water, then elute with 6% ethanol, collect the eluate containing geniposide and genipin-1-β-D-gentiobioside, concentrate and dry the eluent to obtain the geniposide; The total glycosides are 53.3% based on the content of geniposide and genipin-1-β-D-gentiobioside, of which the content of geniposide is 46.2%, and the content of geniposide-1-β-D-gentiobioside The content is 7.1%;

[0076]Or first elute with water and 1% dilute ethanol to remove impurities, then elute with 30% ethanol, collect the eluate containing geniposide and genipin-1-β-D-gentiobioside, wash It is obtained by dehydration, concentration and drying; the total glycosides of geniposide are 51.9% based on the content of ge...

Embodiment 2

[0081] Extract gardenia jasmine with 95% ethanol solution to obtain an ethanol extract, concentrate until it has no alcohol smell; adjust the pH value of the concentrate to 10; separate the solution through 100A macroporous resin column chromatography;

[0082] First remove impurities with water, then elute with 30% ethanol, collect the eluate containing geniposide and genipin-1-β-D-gentiobioside, concentrate and dry the eluate to obtain the geniposide; Total glycosides are greater than or equal to 98.7% based on the content of geniposide and genipin-1-β-D-gentiobioside, of which the content of geniposide is 89.1%, and the content of geniposide-1-β-D-gentian Diglycoside content is 9.6%;

[0083] Or first eluted with water and 5% dilute ethanol to remove impurities, then eluted with 30% ethanol, collected the eluate containing geniposide and genipin-1-β-D-gentiobioside, and washed It is obtained by dehydration, concentration and drying; the total glycosides of geniposide are g...

Embodiment 3

[0088] Extraction process:

[0089] Extract gardenia jasmine with 50% ethanol solution to obtain ethanol extract, concentrate until it has no alcohol smell; adjust the pH value of the concentrate to 8.5; separate the solution through 300 macroporous resin chromatography;

[0090] First remove impurities with water, then elute with 15% ethanol, collect the eluate containing geniposide and genipin-1-β-D-gentiobioside, concentrate and dry the eluate to obtain the product; The total glycosides are 74.2% based on the content of geniposide and genipin-1-β-D-gentiobioside, of which the content of geniposide is 44.2%, and the content of geniposide-1-β-D-gentiobioside The content is 30%;

[0091] Or first elute with water and 3% dilute ethanol to remove impurities, then elute with 20% ethanol, collect the eluate containing geniposide and genipin-1-β-D-gentiobioside, wash It is obtained by dehydration, concentration and drying; the total glycosides of geniposide are 75.7% based on the...

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Abstract

The present invention discloses one kind of total jasminoidin extract and features that the total jasminoidin extract contains jasminoidin 45-90 wt%, genipin-1-beta-D-gentiobioside 5-30 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 50-100 wt% and preferably contains jasminoidin 50-80 wt%, genipin-1-beta-D-gentiobioside 5-25 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 55-100 wt%. Pharmacological experiment shows that the total jasminoidin extract of the present invention has excellent pharmacological effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a gardenia total glycoside extract and a preparation method thereof. Background technique [0002] Gardenia, also known as Gardenia jasminoides, is the fruit of Rubiaceae Gardenia jasminoides Ellis. It is bitter and cold in nature and non-toxic. Gardenia was first recorded in "Shen Nong's Materia Medica", which has the effects of promoting choleresis, stimulating pancreatic secretion, lowering blood pressure, calming, cooling, antibacterial and anti-inflammatory. Gardenia mainly contains iridoid glycosides, organic acids, gardenia yellow pigments and so on. Gardenia iridoid glycosides include geniposide, geniposide, genipin-1-β-D-gentiobioside, gardenic acid, hydroxygeniposide, etc., of which geniposide, genipin-1 -β-D-gentiobioside is a good active ingredient: Chinese patent CN1660198A discloses gardenia iridoid glycosides for the treatment of cholecystit...

Claims

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Application Information

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IPC IPC(8): A61K36/744A61P9/10A61P31/04A61P31/12A61P29/00C07H1/08A61K131/00
Inventor 顾群李志刚魏俊玲郭小鹏渠守峰刘严曹艳芳
Owner PHARMA RES INST OF BENCAO TIANYUAN OF BEIJING
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