Total jasminoidin extract and its prepn process

Abstract

The present invention discloses one kind of total jasminoidin extract and features that the total jasminoidin extract contains jasminoidin 45-90 wt%, genipin-1-beta-D-gentiobioside 5-30 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 50-100 wt% and preferably contains jasminoidin 50-80 wt%, genipin-1-beta-D-gentiobioside 5-25 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 55-100 wt%. Pharmacological experiment shows that the total jasminoidin extract of the present invention has excellent pharmacological effect.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicines, in particular to a gardenia total glycoside extract and a preparation method thereof. Background technique [0002] Gardenia, also known as Gardenia jasminoides, is the fruit of Rubiaceae Gardenia jasminoides Ellis. It is bitter and cold in nature and non-toxic. Gardenia was first recorded in "Shen Nong's Materia Medica", which has the effects of promoting choleresis, stimulating pancreatic secretion, lowering blood pressure, calming, cooling, antibacterial and anti-inflammatory. Gardenia mainly contains iridoid glycosides, organic acids, gardenia yellow pigments and so on. Gardenia iridoid glycosides include geniposide, geniposide, genipin-1-β-D-gentiobioside, gardenic acid, hydroxygeniposide, etc., of which geniposide, genipin-1 -β-D-gentiobioside is a good active ingredient: Chinese patent CN1660198A discloses gardenia iridoid glycosides for the treatment of cholecystit...

AI Technical Summary

Problems solved by technology

The other is the gardenia iridoid glycoside extract, which measures the total amount of geniposide or gardenia iridoid glycosides, and the other main active ingredient of gardenia genipin-1-β- The content of D-gentiobioside has not been determined, resulting in inhomogeneous quality of gardenia extract, its essence is still a mixture, the material basis is not clear, its composition and mutual ratio vary by method, and it is not universal and widely used applicability

[0004] In the prior art, the extraction and purification methods of the total glycosides of gardenia mostly adopt the method of macroporous resin: (1) directly elute with high-concentration ethanol after water elution, this method not only cannot remove the slightly stronger geniposide The iridoid glycoside components of the geniposide, and a lot of weak polar impurities remain [Lv Maoping, Research on the separation effect of geniposide by macroporous resin, Chinese herbal medicine, 2002, 33 (9), 794; Yao Ping, Macroporous adsorption Resin purification of total iridoid glycosides and geniposide in Gardenia, Chinese Herbal Medicine, 2006, 37(1), 57; Chen Gang et al., Research on the purification of Gardenia extract for injection by macroporous adsorption resin, Chinese Journal of Traditional Chinese Medicine, 2005, 30(3), 188; Jin Rixian et al., Study on the process of enriching geniposide in Gardenia jasminoides by macroporous adsorption resin method, Chinese Journal of Experimental Formulas, 2005, 11(3), 1]; (2) Washing with water or eluting with low-concentration ethanol to remove impurities and then eluting with high-concentration ethanol, generally the so-called low-concentration ethanol is 30%, most of geniposide has been removed as impurities, so it is actually impossible to obtain high-quality geniposide The total glycosides extract of the genus glycosides [Chinese patent CN1546507A]; (3) after washing with water, use 10% ethanol to elute to remove impurities, and then use low concentration ethanol 20-30% to elute to obtain [Chinese patent CN1706859A]. In addition to iridoid glycosides, the sub-extract also contains organic acid compounds, such as chlorogenic acid, isochlorogenic acid, etc. Water and 10% ethanol can only elute part of the organic acids, and the other 10% ethanol removes Both genipin-1-β-D-gentiobioside and part of geniposide were eluted, so high content and high yield could not be obtained. Gardenia jasminoides extract of bilebioside; therefore, the prior art does not have a method for obtaining extracts containing high content of geniposide and genipin-1-β-D-gentiobioside, which limits the active substances to a certain extent Application of total glycosides of gardenia in the field of medicine