Reagent kit for detecting pulmonary cancer susceptibility with ERCC2 gene and SNPs site of PARP1 gene

A kit and susceptibility technology, applied in the field of kits for lung cancer susceptibility detection through the SNPs of ERCC2 gene and PARP1 gene, can solve the problems of increased risk and reduced enzyme activity

Inactive Publication Date: 2008-03-12
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Molecular biology studies have shown that amino acid 762 on PARP1 is converted from Val to...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Use of detection kits

[0027]Step 1: Extraction of DNA template

[0028] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0029] Step 2: Real-time quantitative PCR reaction

[0030] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:

[0031] Sense primer 1: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 1) with a Tm value of 58°C

[0032] Antisense primer 1: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 2) with a Tm value of 54°C

[0033] Sense primer 2: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 3) with a Tm value of 58°C

[0034] Antisense primer 2: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 4) with a Tm value of 54°C

[0035] Fluorescent probe 1: 5'-TGCCCAACGAAGTGCTGCAG-3' (SEQ ID NO: 5) with a Tm value of 64°C

[0036] Fluorescent probe 2: 5'-CAGGCCAAGGCGGAAATGCT-3' (SEQ ID NO: 6) with a Tm value of 64°C

[0037] Sense primer 1, antis...

Embodiment 2

[0042] Example 2. Services that guide people to proactively prevent lung cancer

[0043] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.

[0044] Step 1: DNA Extraction

[0045] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.

[0046] Step 2: Genotyping Assays

[0047] Using the kit provided by the invention, simultaneously perform fluorescent quantitative PCR detection on the SNP site rs1799793 on the ERCC2 gene and the SNP site rs1136410 on the PARP1 gene of the DNA of the subject to be tested, and determine the genotypes of the two SNPs sites.

[0048] Step 3: Instruct people to be proactive in preventing lung cancer

[0049] Through the analysis o...

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PUM

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Abstract

The present invention discloses a reagent kit to detect lung cancer susceptibility, which comprises a fluorescence probe to synchronously detect specificity tracers and specificity of No. rs1799793 SNP locus on gene ERCC2 and No. rs1136410 SNP locus on gene PARP1, and a normal assembly to detect fluorescence quantitative PCR and so on. The reagent kit of the present invention synchronously detects and analyzes individual SNPs locus gene carrying type on gene ERCC2 and PARP1 to predict susceptibility of the individual to lung cancer.

Description

technical field [0001] The present invention relates to a kit for detecting disease susceptibility, especially a kit for detecting susceptibility to lung cancer, by simultaneously detecting Excision Repair Cross-complementing Rodent Repair Deficiency, Complementation Group 2, ERCC2 The single nucleotide polymorphism (SNP) site and poly ADP-ribose transferase family member 1 (poly (ADP-ribose) polymerase family, member 1, PARP1, also abbreviated as ADPRT) single nucleotide polymorphism Loci to predict an individual's susceptibility to lung cancer. Background technique [0002] Primary bronchial lung cancer, referred to as lung cancer, is one of the most common malignant tumors in the world and a serious threat to human health and life. Tumor cells originate from the bronchial mucosa or glands, and often have regional lymph node and hematogenous metastasis. In the early stage, respiratory symptoms such as irritating cough and blood in the sputum are often present. The speed o...

Claims

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Application Information

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IPC IPC(8): G01N21/64C12Q1/68
Inventor 冯哲民邹祖烨
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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