Reagent kit for detecting pulmonary cancer susceptibility with ERCC2 gene and SNPs site of PARP1 gene
A kit and susceptibility technology, applied in the field of kits for lung cancer susceptibility detection through the SNPs of ERCC2 gene and PARP1 gene, can solve the problems of increased risk and reduced enzyme activity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0026] Example 1. Use of detection kits
[0027]Step 1: Extraction of DNA template
[0028] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.
[0029] Step 2: Real-time quantitative PCR reaction
[0030] Use a fluorescent quantitative PCR detection kit that can detect susceptibility to lung cancer, which contains the following primer pairs and fluorescent probes:
[0031] Sense primer 1: 5'-ATCAAAGAGACAGACGAGCA-3' (SEQ ID NO: 1) with a Tm value of 58°C
[0032] Antisense primer 1: 5'-TCAGGAAGCCCAGGAAAT-3' (SEQ ID NO: 2) with a Tm value of 54°C
[0033] Sense primer 2: 5'-TTTGCCATTCACTGTGTTGG-3' (SEQ ID NO: 3) with a Tm value of 58°C
[0034] Antisense primer 2: 5'-ATCCTTGCTGCTATCATCA-3' (SEQ ID NO: 4) with a Tm value of 54°C
[0035] Fluorescent probe 1: 5'-TGCCCAACGAAGTGCTGCAG-3' (SEQ ID NO: 5) with a Tm value of 64°C
[0036] Fluorescent probe 2: 5'-CAGGCCAAGGCGGAAATGCT-3' (SEQ ID NO: 6) with a Tm value of 64°C
[0037] Sense primer 1, antis...
Embodiment 2
[0042] Example 2. Services that guide people to proactively prevent lung cancer
[0043] At present, Shanghai Zhujian Bioengineering Co., Ltd. has launched this service.
[0044] Step 1: DNA Extraction
[0045] The subjects were consulted before the service, and the subjects signed the informed consent and filled out the questionnaire on living and eating habits. According to the instructions in the sampling box, the oral epithelial cells were sampled with oral swabs, and the DNA of the oral epithelial cells was extracted by silica gel adsorption.
[0046] Step 2: Genotyping Assays
[0047] Using the kit provided by the invention, simultaneously perform fluorescent quantitative PCR detection on the SNP site rs1799793 on the ERCC2 gene and the SNP site rs1136410 on the PARP1 gene of the DNA of the subject to be tested, and determine the genotypes of the two SNPs sites.
[0048] Step 3: Instruct people to be proactive in preventing lung cancer
[0049] Through the analysis o...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com