Novel technical method for DNA and protein interaction research
A technical solution, protein extraction technology, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve the problems of injury, radionuclide pollution, cumbersome experimental process, etc.
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[0061] Example 1, the following is a study on the combination of the DNA fragment of the 5' untranslated region of P53 and the Nucleol in nucleoprotein after radiation intervention, using the technical scheme of the present invention, to achieve the expected experimental purpose .
[0062] 1. Materials and methods
[0063] 11 Instruments and Reagents
[0064] 1.1.1 Cell line: MCF7 human breast cancer cell line was obtained from Basic Immunology Laboratory.
[0065] 1.1.2 Irradiation equipment: domestic X.S.S.205 (FZ) fixed X-ray deep therapy machine.
[0066] 1.13 Reagent preparation
[0067] (1) Tris-SDS-glycine electrophoresis buffer
[0068] (2) Tris-SDS-glycine-methanol transfer buffer
[0069] (3) 5×Banding Buffer (Glycerol, MgCl 2 , EDTA, DTT, NaCl, Tris-HCL, Poly DI-DC)
[0070] 1.2 Experimental part
[0071] 1.2.1 Acquisition of target DNA fragments
[0072] According to the p53 tumor suppressor mRNA sequence design the PCR primers of p53 gene (synthesized by Li...
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