Novel technical method for DNA and protein interaction research

A technical solution, protein extraction technology, applied in the direction of material inspection products, measuring devices, instruments, etc., can solve the problems of injury, radionuclide pollution, cumbersome experimental process, etc.

Inactive Publication Date: 2008-03-19
JILIN UNIV
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  • Abstract
  • Description
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Problems solved by technology

[0003] However, in the traditional test scheme, the experimental process is often cumbersome because the DNA prob

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  • Novel technical method for DNA and protein interaction research

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Embodiment 1

[0061] Example 1, the following is a study on the combination of the DNA fragment of the 5' untranslated region of P53 and the Nucleol in nucleoprotein after radiation intervention, using the technical scheme of the present invention, to achieve the expected experimental purpose .

[0062] 1. Materials and methods

[0063] 11 Instruments and Reagents

[0064] 1.1.1 Cell line: MCF7 human breast cancer cell line was obtained from Basic Immunology Laboratory.

[0065] 1.1.2 Irradiation equipment: domestic X.S.S.205 (FZ) fixed X-ray deep therapy machine.

[0066] 1.13 Reagent preparation

[0067] (1) Tris-SDS-glycine electrophoresis buffer

[0068] (2) Tris-SDS-glycine-methanol transfer buffer

[0069] (3) 5×Banding Buffer (Glycerol, MgCl 2 , EDTA, DTT, NaCl, Tris-HCL, Poly DI-DC)

[0070] 1.2 Experimental part

[0071] 1.2.1 Acquisition of target DNA fragments

[0072] According to the p53 tumor suppressor mRNA sequence design the PCR primers of p53 gene (synthesized by Li...

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Abstract

The present invention relates to a new technical proposal for testing, diagnosing, and studying the biology and the medicine. The present invention is characterized in that the cell protein extract combines into compound earlier than the corresponding antibody molecule, and combines with the null probe into a triple compound of the antibody-albumen-null probe, which can be moved into the non-metamorphic polyacrylamide gel for electrophoresis, and finally an ECL method is used for the irradiance test in darkroom. Compared with the traditional method, the technology has simple and reliable operation without radionuclide element pollution.

Description

technical field [0001] The invention relates to the field of molecular biology research, in particular to the innovation of experiment technology in the research of the interaction between DNA binding protein and related biological probes. Background technique [0002] During the life activities of cells, the replication and recombination of DNA, the transcription and modification of RNA, and the infection and proliferation of viruses all involve the interaction between specific DNA segments and special protein binding factors. Therefore, people have been paying attention to the study of DNA-binding proteins for a long time. Especially since the development of recombinant DNA technology, a large number of genes with biological significance have been isolated one after another. Under the support of this background, people began to explore how external environmental factors and developmental signals affect or regulate the transcriptional activity of genes. topic. Gel retarda...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/561
Inventor 刘晓东赵银龙李文兴
Owner JILIN UNIV
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