Sensing element for detecting total cholesterol in a blood sample

Abstract

A sensor for detecting total cholesterol in a blood sample, comprising a working electrode and a counter electrode respectively formed on a first substrate and a second substrate, one of the first and second substrates has an opening opened in the The position where the electrode is formed allows the blood sample to pass through; the electrode formed on the surface of the first or second substrate and exposes the insulating layer of the electrode terminal joint of the substrate; the reaction pad that is arranged between the working electrode and the counterpart electrode and contains the reaction reagent and a separation pad arranged on the electrode opening of the substrate, before the blood sample enters the electrode opening, the interference in the specimen is separated and removed through the separation pad. The sensor is provided with a separation pad at a specific position to effectively remove blood cells and interfering macromolecules in the blood sample, so as to achieve the purpose of rapid and accurate detection of total cholesterol in the whole blood sample.

Description

technical field [0001] The invention relates to a sensor for detecting total cholesterol in a blood sample, in particular to a sensor for electrochemically detecting the total cholesterol in a blood sample. Background technique [0002] The total cholesterol content in the blood is one of the indicators of blood lipid testing, and is often used as the basis for early prevention and / or treatment evaluation of arteriosclerosis and cardiovascular diseases. If it can be detected at any time in daily life and the cholesterol content in the blood is controlled within the standard value, it can effectively prevent arteriosclerosis and reduce the incidence of cardiovascular diseases. [0003] In the implementation of the measurement of biochemical molecular components, in the past, spectrophotometers can be used to combine reaction components to perform optical color reaction analysis at specific wavelengths, such as absorbance. However, there are limitations that analytical instru...

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Problems solved by technology

However, the principle of this kind of horizontal chromatography requires a long time for sample separation, and it is difficult to control the moving speed of blood cells. It cannot ensure that blood cells will not enter the reaction zone at all, and it still cannot meet the needs of rapid and accurate detection.

On the other hand, Japanese Laid-Open Patent No. 2004-245736 discloses a test strip that can detect high-density cholesterol (HDL) and triglyceride using whole blood, but the structure of the test strip disclosed in this patent is relatively complicated. To use a blood cell delay agent, and the blood cell filtration and plasma flow must rely on external assistance to pump air, a sample of up to tens of μL is required and the reaction time is as long as tens of minutes

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