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Protankyra bidentata anti-tumor factor and its composition, preparation method and application

An anti-tumor and thorn-thorn technology is applied in the field of biomedicine to achieve significant curative effects, inhibit growth and reproduction, and achieve obvious effects.

Inactive Publication Date: 2008-04-23
NAVY MEDICINE RES INST OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Modern medical research has shown that sea spiny ginseng is rich in various proteolytic enzymes, and there are no other research reports

Method used

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  • Protankyra bidentata anti-tumor factor and its composition, preparation method and application
  • Protankyra bidentata anti-tumor factor and its composition, preparation method and application
  • Protankyra bidentata anti-tumor factor and its composition, preparation method and application

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Experimental program
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Effect test

Embodiment 1

[0047] Freshly frozen marine anchor ginseng, extracted with 10mmol / L PB containing 1mol / L NaCl for 24h, 8000rpm high-speed frozen centrifugation, the supernatant was used (NH 4 ) 2 SO 4 Fractional precipitation, the precipitation fraction with a saturation of 40% to 60% was subjected to DEAE-Sepharose CL-6B ion exchange chromatography, and linear gradient elution was performed with PBS buffer containing 0 to 1mol / L NaCl, flow rate: 30ml / h , collect 0.45mol / L~0.65mol / L NaCl gradient active components (the second peak, as shown in Figure 1), carry out Sephacryl-300 column (1.6 * 50cm), eluent: 10mmol / L PB, pH7. 2 buffer solution, flow rate: 20ml / h, collect the active peak (the first peak, as shown in Figure 2), and concentrate the components for HPLC column: ZOR BAX GF-250, 9.4 × 250mm, 4-Micron, elution Solution: 10mmol / L PB, flow rate: 1ml / min, column temperature: 20°C, collect activity peaks (as shown in Figure 3), and obtain PBATF after lyophilization.

Embodiment 2

[0049] Freshly frozen marine anchor ginseng, extracted with 4 times the volume of 15mmol / LPB buffer solution containing 2mol / L NaCI for 24h, 10000rpm high-speed frozen centrifugation, the supernatant was used (NH 4 ) 2 SO 4 Fractional precipitation, the precipitation fraction with a saturation of 40% to 60% was subjected to DEAE-Sephadex A 50 ion exchange chromatography, and linear gradient elution was performed with 15 mmol / L PB buffer containing 0 to 1 mol / L NaCl, and 0.45 mol / L~0.65mol / L NaCl gradient active components were subjected to Sephadex G-100 molecular sieve chromatography, the active peaks were collected, and the concentrated components were subjected to HPLC chromatography (ZORBAX GF-250, 9.4×250mm, 4-Micron), The active peaks were collected and freeze-dried to obtain PBATF.

Embodiment 3

[0051] Freshly frozen marine anchor ginseng was extracted with 3 times the volume of 20mmol / LPB buffer solution containing 1.5mol / L NaCI for 24h, 10000rpm high-speed frozen centrifugation, and the supernatant was used (NH 4 ) 2 SO 4 Fractional precipitation, the precipitation fraction with a saturation of 40% to 60% was subjected to DEAE-Sephadex A 50 ion exchange chromatography, and 20mmol / L PB buffer containing 0 to 1mol / L NaCl was used for linear gradient elution, and 0.45 mol / L~0.65mol / L NaCl gradient active components were subjected to Sephadex G-150 molecular sieve chromatography, the active peaks were collected, and the concentrated components were subjected to HPLC chromatography (ZORBAX GF-250, 9.4×250mm, 4-Micron), The active peaks were collected and freeze-dried to obtain PBATF.

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Abstract

The present invention relates to biomedicine technology, and is especially Stichopus japonicus antitumor factor, and its composition, preparation process and application. The Stichopus japonicus antitumor factor is prepared with Stichopus japonicus as material, and through extraction, salting out, ion exchange chromatography, molecular sieve chromatography and HPLC purification. It is protein with antitumor activity and in the molecular weight of 38 kD. It can inhibit the growth and propagation of tumor cell obviously, no obvious toxic side effect, and may be applied as the active component for antitumor medicine.

Description

Technical field: [0001] The invention relates to the field of biomedicine, in particular to an anti-tumor factor of spiny ginseng, its composition, preparation method and application. Background technique: [0002] Protankyra bidentata is a marine echinoderm; it is thin cylindrical, looks like a worm, has no tube feet and warty feet, and has 12 tentacles. The bone fragments are anchors and anchor plates; they grow from the coarse sand in the intertidal zone of the ocean to the mud bottom with a water depth of 15 meters, and the body is fleshy red. The spiny anchor ginseng grows in the coastal areas of our country, and the reserves are huge. It is salty and cold in nature, rich in protein, and extremely delicious in taste. It has the effects of nourishing yin and reducing fire, tonifying deficiency and clearing lung, lowering blood pressure, reducing swelling and pain, and preventing cancer. It has high medicinal and dietary value. [0003] A variety of herbal medicines in ...

Claims

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Application Information

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IPC IPC(8): C07K4/12C07K14/435A61K38/17A61P35/00A61P35/02
Inventor 沈先荣栾杰蒋定文陈伟贾福星谷爱梅
Owner NAVY MEDICINE RES INST OF PLA
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