Technique for concentrating chicken ND-IB-AI-EDS tetrad oil emulsion killed vaccine antigen

Abstract

The invention provides a concentration technology of virus antigen during the preparation process of a chicken ND-IB-AI-EDS quadruple inactivated oil-emulsion vaccine. The main contents of the method are that: newcastle disease virus (NDV), infectious bronchitis virus (IBV) and H9 subtype avian influenza virus (AIV) are concentrated by four steps of rough filtration, fine filtration, ultrafiltration and material collection via an internal pressure hollow fiber ultrafilter. After the concentration, the three virus antigens are mixed with egg drop syndrome virus (EDSV) for the preparation of the chicken ND-IB-AI-EDS quadruple inactivated oil-emulsion vaccine.

Description

technical field

[0001] The method is a technique for concentrating virus antigens during the preparation of chicken ND-IB-AI-EDS quadruple oil emulsion inactivated vaccine. Background technique

[0002] With the development of my country's chicken industry, the breeding scale and breeding density are increasing. Due to the long breeding cycle of laying hens and breeders, the risk of being attacked by diseases is also high, forcing farms to frequently vaccinate chickens with different The single seedling of different species not only consumes a lot of manpower and material resources, but also causes severe stress response to the flock and affects the production performance. Double, triple and multiple vaccines can prevent two, three or even multiple diseases at one time, making vaccination easier and more economical. They are used more and more in production practice and are loved by farmers. . In the production of multiple vaccines, whether the antigen content per unit volu...

Examples

example 1

[0017] Example 1 Poultry ND-IB-AI-EDS quadruple oil emulsion inactivated vaccine virus antigen enrichment and immune comparison test

[0018] 1. Materials and methods

[0019] 1.1 Virus liquid: NDV, IBV, AIV (H 9 subtype), EDS live virus liquid and inactivated virus liquid are provided by our laboratory.

[0020] 1.2 Main instruments and equipment: mesoporous fiber ultrafilter, produced by Tianjin Polytechnic University Membrane Separation Technology Research Institute; colloid mill, product of Shenyang Xinguang Machinery Factory; homogenizer, purchased from Shanghai Huadong Homogenizer Factory.

[0021] 1.3 Inactivation pre-virus antigen concentration test: NDV, IBV, AIV (H 9 ) is concentrated 4 times, calculate the concentration time and the volume of filtered water, and collect the virus samples and isobaric flushing water and backwash water samples before and after concentration respectively, and apply the micro-method to measure the HA titer of the samples to 1% chicken...

technical field

[0001] The method is a technique for concentrating virus antigens during the preparation of chicken ND-IB-AI-EDS quadruple oil emulsion inactivated vaccine. Background technique

[0002] With the development of my country's chicken industry, the breeding scale and breeding density are increasing. Due to the long breeding cycle of laying hens and breeders, the risk of being attacked by diseases is also high, forcing farms to frequently vaccinate chickens with different The single seedling of different species not only consumes a lot of manpower and material resources, but also causes severe stress response to the flock and affects the production performance. Double, triple and multiple vaccines can prevent two, three or even multiple diseases at one time, making vaccination easier and more economical. They are used more and more in production practice and are loved by farmers. . In the production of multiple vaccines, whether the antigen content per unit volu...

Examples

example 1

[0017] Example 1 Poultry ND-IB-AI-EDS quadruple oil emulsion inactivated vaccine virus antigen enrichment and immune comparison test

[0018] 1. Materials and methods

[0019] 1.1 Virus liquid: NDV, IBV, AIV (H 9 subtype), EDS live virus liquid and inactivated virus liquid are provided by our laboratory.

[0020] 1.2 Main instruments and equipment: mesoporous fiber ultrafilter, produced by Tianjin Polytechnic University Membrane Separation Technology Research Institute; colloid mill, product of Shenyang Xinguang Machinery Factory; homogenizer, purchased from Shanghai Huadong Homogenizer Factory.

[0021] 1.3 Inactivation pre-virus antigen concentration test: NDV, IBV, AIV (H 9 ) is concentrated 4 times, calculate the concentration time and the volume of filtered water, and collect the virus samples and isobaric flushing water and backwash water samples before and after concentration respectively, and apply the micro-method to measure the HA titer of the samples to 1% chicken...

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