Method for extracting flavonoid from Sarcopyramis nepalensis Wall

Abstract

The invention relates to a method for extracting flavonoid from Sarcopyramis nepalensis Wall. The dry matter of the overground part of a Sarcopyramis nepalensis Wall plant is crushed and then is heated and refluxed for three times by using alcohol of 95 percent as solvent after being cold-dipped in the alcohol of 95 percent for 24 hours; filter liquor is collected to a vacuum rotation concentration instrument to be concentrated into extract. The extract is suspended by using the water which is one time the volume of the extract, and is extracted by using ethyl acetate which is twice the volume of the suspending liquid; the extraction is carried out for three to five times, extracting filter liquor is merged in the vacuum rotation concentration instrument to be concentrated into the extract. The extract is dissolved by using the ethyl acetate; the dissolved solution is led to a silica gel column with 200 to 300 screen meshes, and is eluted in a gradient way by using sherwood oil and the ethyl acetate; the proportion of the sherwood oil and the ethyl acetate is 5:1; the eluent, in which the proportion of the sherwood oil and the ethyl acetate is 2:1, is collected, concentrated in a vacuum rotation way, dissolved by hot absolute alcohol, crystallized by using cold acetone, and recrystallized by using methanol to obtain flavonoid matter crystal in the Sarcopyramis nepalensis Wall plant.

Description

Technical field [0001] The invention relates to an extraction method for extracting flavonoids from plants. technical background [0002] S. nepalensis Wall., belongs to Sarcopyramis Wall. in Melastomaceae, and is also known as "Sarcopyramis Wall." It is an annual herb, 10-20cm high, the stem is fleshy and hairless, with four edges, and the whole plant is purple-red. The leaf pair grows ovate or lanceolate, 3-6cm long, 1.0-2.5cm wide, apex is long and acuminate, edge is serrated, 3-5 main veins, long stalk, leaf surface is blue-green, leaf back is light green , Is white sparse bristles. The flowers are bisexual, light yellow or purple, with several clusters, branch tops or leaf axils; the calyx is inverted spire-shaped, shaped like a wind chest, hence the name. "The Dictionary of Chinese Medicine" records: its nature is cool, tastes sour, non-toxic; functions: clear lung heat, remove hepatitis; cure rheumatism arthralgia, tinnitus, deafness, and clear eyesight. "Practical Chinese...

AI Technical Summary

Problems solved by technology

[0003] So far, there is no report about the extraction of flavonoids from Chutouhong plants, and it is the first time that flavonoids have been isolated from Chutouhong plants at home and abroad.