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A technology of functional bacteria and microbial strains, applied in the field of microorganisms, can solve the problems of long recovery period of engineering bacteria, poor manganese removal effect, long start-up time, etc., and achieve the effect of shortening the establishment time, accelerating the filtration speed and shortening the recovery period
Inactive Publication Date: 2008-09-10
HARBIN INST OF TECH
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[0009] The purpose of the present invention is to solve the problem that the current bio-oxidative manganese removal engineering flora is established by natural selection, the biochemical ability is weak, the mang
Method used
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specific Embodiment approach 1
[0017] Specific Embodiment 1: In this embodiment, the biological manganese and iron removal functional bacterium MS604 (Sphingobacterium sp.) belongs to the genus Sphingobacterium, and was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation date was October 30, 2007 , the preservation number is CGMCCNo.2240; the biological manganese and iron removal function bacterium MS604 (Sphingobacterium sp.) is a Gram-negative aerobic bacterium, a polymorphic bacillus, with a length of 1-2 μm, a width of 0.3-0.4 μm, and two cells. The end is round and blunt; milky yellow colonies are formed on the medium, the colonies are round, opaque, and the edges are neat; the biological manganese and iron removal function bacterium MS604 (Sphingobacterium sp.) has energy storage particles, spores and capsules (such as figure 2 and Figure 4 shown), N, N, N', N'-tetramethyl-p-phenylenediamine (N, N, N', N'-Tetramethyl-p-P...
specific Embodiment approach 2
[0020] Specific embodiment two: In this embodiment, the biological manganese and iron removal functional bacterium MS604 (Sphingobacterium sp.) is obtained by screening from the mud taken from the aeration tank wall of the biological manganese removal filter aeration tank of Zhangshi biological manganese removal water plant in Shenyang City, Liaoning Province . 1L medium for screening consists of 0.8g peptone, 0.2g yeast extract, 0.1g MnSO 4 .H 2 O, 0.1 g K 2 HPO 4 , 0.2g MgSO 4 ·7H 2 O, 0.2g NaNO 3 , 0.1g CaCl 2 , 0.1g (NH 4 ) 2 CO 3 , 0.01g FeSO 4 ·7H 2 O and the remainder of distilled water; then sterilized.
[0021] The present embodiment adopts the combination of plate streaking and plate coating method to divide bacteria, and adopts TMPD method (Vandenabeele J, D de Beer et al. Manganese oxidation by microbial consortia from sand filters. Microb. Ecol. 1992, 24: 91-108. ) for the primary screening of the isolated strains. The principle of using the TMPD met...
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Abstract
The invention relates to a biological demanganization and deferrization functional bacterium, which relates to a microorganism. The invention solves the problems that: the prior biological oxidation demanganization engineering flora is established in virtue of natural selection, thereby the biochemical capacity is weak and the demanganization effect is bad; the culture, acclimation and starting time required for a water treatment demanganization biological filter chamber is long, and the restore cycle after the enginerring flora is damaged is long. The biological demanganization and deferrization functional bacterium MS604 (Sphingobacterium sp.) belongs to Sphingobacterium sp. and is preserved in Common Microorganism Center of China Committee for Culture Collection of Microorganisms; the preservation date is October 30th, 2007 and the preservation No. is CGMCC No.2240; the strain MS604 is a gram negative aerobic bacterium and a polymorphous bacillus with a length of 1 to 2 micrometers and a width of 0.3 to 0.4 micrometers, and both ends of cells are round and obtuse; a milk yellow colony is formed on a culture medium and is round and opaque with tidy edges; the strain MS604 is provided with energy storage particles and also provided with a brood cell and a capsule, and N, N, N', N'-tetramethyl-p-phenylenediamine is dyed to be masculine. The biological demanganization and deferrization functional bacterium MS604 has the capacity for removal of Mn<2+> and Fe<2+> in water; the removal rate of Mn<2+> is higher than 79 percent and the removal rate of Fe<2+> is higher than 90 percent.
Description
technical field [0001] The present invention relates to a microorganism. Background technique [0002] The iron removal technology in water is relatively mature, and there are many engineering bacteria that can only remove iron. However, iron and manganese are abundant metal elements in the earth's crust, and the removal of iron alone is far from meeting the existing water treatment standards. The concentration of manganese ions in ordinary water is 0.1-1 mg / L, while the concentration of soluble manganese ions in anaerobic groundwater can be as high as 7-9 mg / L. Soluble manganese can form insoluble precipitates (such as MnO 2 or MnOOH). [0003] MnO 2 is a highly insoluble substance (δ-MnO 2 The solubility kso is only 10 -56 ), which is dark black and can cause significant coloration in groundwater intended for drinking; manganese must therefore be removed from drinking water. my country stipulates that the maximum concentration of manganese ions in drinking water is 0...
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