Lei bamboo seed embryo culture medium and tissue-culture seedlings-cultivation method

A technology for culture medium and bamboo seeds, which is applied in the directions of horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of bamboo farmers who cannot provide sufficient bamboo seedlings, and achieves low cost, convenient transportation, and is not affected by seasons. The effect of restriction

Inactive Publication Date: 2009-01-07
ZHEJIANG FORESTRY UNIVERSITY
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Problems solved by technology

[0003] The present invention aims at the actual situation that the tissue culture seedlings of Phyllostachys praecox have not been successfully cultivated so far, and there is a shortcoming that the bamboo farmers who are in urgent need of Phyllostachys

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  • Lei bamboo seed embryo culture medium and tissue-culture seedlings-cultivation method

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Embodiment Construction

[0012] The present invention is further described below in conjunction with the examples: the raw materials and proportioning that culture medium selects are except MS salts, MT vitamins, also have BA (0,0.3,1,3mg / L), NAA (0,0.05,0.1 . MT, nutrient, and coagulant are the best fixed additions (the additions of MS and MT are carried out according to the product instructions), and both BA and NAA have two situations of not adding and adding. BA also has three different dosages of 0.3, 1, and 3 mg / L; NAA has three different dosages of 0.05, 0.1, and 1 mg / L; amiloride has three different dosages of 0.05, 0.1, and 0.5; TDZ has 0.0001, 0.001, and 0.01 were added in three different amounts, and the pH value of the medium also had four situations of 5, 5.2, 5.7, and 6, respectively. Therefore, a variety of formulas were combined and placed in 160 test tubes respectively. Embryos were subjected to tissue culture, and the conditions of rooting, germination and browning were shown in Tab...

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Abstract

The invention provides an embryo culture medium for Lei bamboo (Phyllostachys praecox), which comprises the following components by weight part: MS salts, MT vitamins, 0.3 mg/L or 1 mg/L or 3 mg/L of BA (i.e. benzylamino purine), 0.1 mg/L of NAA (i.e. alpha-naphthaleneacetic acid), 0.1 mg/L of 3,5,6-trichloro-4-aminopicolinic acid, 0.001 mg/L of TDZ (i.e. thidiazuron), 20 g/L of glucose, 100 mg/L of inositol, 10 mg/L of adenine sulfate, and 0.5 g/L of malt extract. The pH value of the culture medium ranges from 5.0 to 6.0. The method of tissue culture and seedling cultivation using the culture medium comprises the following steps: collection and selection of explants, cleaning and sterilization of seeds, and extraction, inoculation, culture, sub-culture or transplanting of the embryos. The culture medium and the seedling tissue culture method are excellent in the cultivation of the Lei bamboo as the medium-diameter running bamboo with high economic value, lay the foundation for researching and bioengineering of Lei bamboo, and realize the rapid and sufficient supply of Lei bamboo seedlings.

Description

【Technical field】 [0001] The invention relates to a medium for biological tissue and a tissue culture method. 【Background technique】 [0002] Thunder bamboo (Phyllostachys praecox), also known as early bamboo, early garden bamboo, Lei Gongzhu, is a bamboo species of the genus Bamboo in the Poaceae Bamboo subfamily. Thunder bamboo is an excellent bamboo species for bamboo shoots. The annual output of bamboo shoots per mu is as high as 2 tons, and the annual output value per mu exceeds 20,000 yuan. Through mulching, fertilization, and temperature-increasing measures, not only the production of Phyllostachys praecox is significantly increased, but also the bamboo shoots can emerge in advance in the winter before the Spring Festival, providing the market with fresh bamboo shoots that people crave, and becoming a popular New Year product and gift. Phyllostachys praecox has strong adaptability, and it is suitable to be planted in low hills and gentle slopes, land in front of and ...

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Application Information

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IPC IPC(8): A01H4/00A01G31/00C12N5/04
Inventor 林新春方伟桂仁意裴海燕朱圆圆黄丽春
Owner ZHEJIANG FORESTRY UNIVERSITY
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