Compositions and methods for modulating the immune system
A composition and compound technology, applied in the direction of drug combination, active ingredients of heterocyclic compounds, blood diseases, etc., can solve the problem of long cost
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Embodiment 1
[0367] Materials and methods
[0368] Riboflavin, nitroblue tetrazolium (NBT), reduced glutathione (GSH), S-S′-dithiobis(2-nitrobenzoic acid) (DTNB), and 1-chloro-2 , 4-Dinitrobenzene (CDNB) was obtained from Sisco Research Laboratories Pvt. Ltd, Mumbai, India. Thiobarbituric acid was purchased from Hi-media laboratories, Mumbai, India, and 1,1,3,3-tetramethoxypropane was supplied by Sigma-Aldrich USA. All other chemicals and reagents used in this study were of analytical grade. Oltipraz was supplied by contractor Canopus Corporation.
[0369] Inbred 4-6 week old female Swiss albino mice (20-30 g) were obtained from a small animal vivarium, Kerala Agricultural University, Mannuthy, Thrissur. Animals were maintained in well-ventilated cages under standard conditions of temperature, pressure and humidity. Animals were provided normal mouse chow and water ad libitum. All animal experiments performed during this study were pre-approved and followed the guidelines of the Ins...
Embodiment 2
[0383] Example 2 - Patient Case Study
[0384] Patients are treated with the compound of formula 2 and blood cell parameters are monitored. The experiment has a sample size of 1 and consists of subject 1. Subject 1, an immunocompromised patient who suffered from multiple recurrent infectious episodes requiring hospitalization, was initiated in a near-moribund state with the experimental drug isopentenyladenosine (IPA).
[0385] The subject has been hospitalized for a life-threatening infection. During the three months prior to hospitalization, the subject's total white blood cell count was in the range of 2200-4900. A T4 / T8 cell ratio of 50 / 270 (0.22) was observed during the first two months. The patient was hospitalized with depression, exhaustion, bronchial infection, severe diarrhea, severe weight loss, and complete loss of appetite and intensified fever (high fever, spiked fever). The patient weighed 98 lbs.
[0386] Although initially very cooperative for patients ...
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