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Reagent kit for detecting cigarette and wine damnification genetic susceptibility

A kit and susceptibility technology, applied in the fields of molecular biology and medicine, can solve problems such as alcoholism, discomfort, and decreased metabolism of acetaldehyde

Inactive Publication Date: 2009-01-28
XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In contrast, the risk of Alzheimer's disease among people who tested negative for the e4 allele of the APOE gene was less than 10%.
The rs671 polymorphic change (G / A) defect of the ALDH2 gene leads to a decrease in the body's ability to metabolize acetaldehyde, causing discomfort in drinking, flushing syndrome, etc., and easily leading to alcoholism

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1. Use of detection kits

[0015] Step 1: Extraction of DNA template

[0016] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0017] Step 2: Real-time quantitative PCR reaction

[0018] Using the detection kit, an independent fluorescent quantitative PCR reaction was carried out for each SNP site. The total volume of each reaction was 10 μl, including 2 μl of DNA template with a concentration of 20 ng / μl, 1 μl of 10X fluorescent quantitative PCR reaction buffer, 0.1μl 25mM dNTP mixture, 0.6μl 25mM MgCl 2 solution, 0.025 μl (5 units / μl) Taq DNA polymerase, 0.225 μl each of 20 μM primers, 0.25 μl each of 10 μM fluorescent probes, and 5.325 μl of deionized water.

[0019] The reaction was carried out on an ABI9700 type PCR amplification instrument, and the reaction conditions were 50° C. for 2 minutes, 95° C. for 10 minutes, and 60 cycles of 95° C. for 30 seconds and 60° C. for 1 minute. After the reaction, the fluorescence of the...

Embodiment 2

[0022] Example 2. Services for testing genetic susceptibility to tobacco and alcohol damage

[0023] Step 1: DNA Extraction

[0024] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0025] Step 2: Genotyping Assays

[0026] Use the kit provided by the present invention to perform fluorescence on 16 SNP sites on the ADH2, ALDH2, CYP1A1, CYP2A13, CYP2E1, ENOS, ERCC2, GSTM1, GSTP1, GSTT1, MTHFR, NQO1 and XRCC1 genes of the genomic DNA of the subject respectively Quantitative PCR was used to determine the genotypes of these SNPs.

[0027] Step 3: Analysis of genetic susceptibility to tobacco and alcohol injury

[0028] Through the analysis of the SNPs genotypes of the subjects, an analysis report sheet of genetic susceptibility to tobacco and alcohol injury is issued, and the doctor explains and interpre...

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PUM

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Abstract

The invention discloses a kit that is used for detecting the genetic predisposition of wine and tobacco damages. The kit comprises particularity primer pairs and particularity fluorescent probe pairs that are used for simultaneously detecting 16 SNP polymorphism genotypes on the genes of ADH2, ALDH2, CYP1A1, CYP2A13, CYP2E1, ENOS, ERCC2, GSTM1, GSTP1, GSTT1, MTHFR, NQO1 and XRCC1 and a routine component that is used for fluorescent quantitative PCR detection, etc. The kit of the invention evaluates the genetic predisposition of wine and tobacco damages by simultaneously detecting the 16 mononucleotide polymorphism locus genotypes that are closely related with the genetic predisposition of wine and tobacco damages.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, more specifically, the present invention relates to a kit for detecting genetic susceptibility to tobacco and alcohol injury, which can be used to assess individual tobacco and alcohol injury by detecting and analyzing genes related to the genetic risk of tobacco and alcohol injury genetic predisposition. Background technique [0002] Genetic detection and analysis technology has rapidly become an important tool for predicting human disease and health. Gene technology can correlate human health status with physiological diseases, genetic mutation factors, disease tendency and status, etc. Genetic factor mutations caused by base insertions, deletions, and mismatches in DNA sequences, as well as up-and-down regulation of individual DNA expressions, are all biological information related to health status and disease mechanisms. [0003] For example, "senile dementia" is a per...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/00G01N33/50C12Q1/68
Inventor 邹祖烨冯哲民
Owner XINBAXIANG SHANGHAI MOLECULAR MEDICAL TECH SHANGHAI
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