Purification cultivation method of human prolactinomas cell

Abstract

The invention relates to a method for the pure culture of human pituitary prolactinoma cells, which is characterized in that 'moderate enzyme digestion and filtration' is adopted to remove fibroblasts and 'red blood cell lysis solution' is adopted to remove red blood cells, and except the two kinds of the cells, the remained cells are human pituitary prolactinoma cells, wherein the method comprises the following steps: (1), putting tumor tissues into culture solution; (2), cutting tissue blocks into small pieces; (3), digesting the small pieces by pancreatins; (4), filtering the digested substance through a cell sieve and terminating the digestion; (5), adding the red blood cell lysis solution and re-suspending the cells; (6), removing the red blood cell lysis solution, (7), absorbing the culture solution and adding the culture solution into a blood cell counting plate, then counting; and (8), inoculating the cells into a 24-hole culture plate by each hole having 1 x 10<6> cells, and putting the culture plate in a cell incubator. The mentod is easy and convenient to operate and can simultaneously remove fibroblasts and the red blood cells, the obtained pituitary adenoma cells are authenticated through an immunocytochemistry method, and the cell purity is high and is close to 100 percent.

Description

technical field [0001] The invention belongs to the field of medical science and technology, in particular to a method for purifying and culturing human pituitary prolactinoma cells. Background technique [0002] Pituitary adenoma is one of the common intracranial tumors, and the highest incidence rate is prolactinoma, accounting for 40% to 60%. The clinical manifestations of prolactinoma include endocrine symptoms and mass symptoms. The former is mainly caused by hyperprolactinemia, including amenorrhea, lactation, infertility, sexual dysfunction, etc., and the latter is headache, nausea, vomiting, vision loss, etc. . The pathogenesis, biological characteristics, and drug screening of the disease depend on the purification and culture of tumor cells, and there are many purification and culture methods seen in the literature, such as the use of the nutritional dependence of pituitary adenoma cells and fibroblasts and It takes about 6 days to separate the cells with differe...

AI Technical Summary

Problems solved by technology

However, these methods cannot obtain satisfactory results.