Composite premixing feedstuff capable of suspending sow physiological function decline
A physiological function and feed technology, applied in the direction of animal feed, animal feed, additional food elements, etc., can solve the problems of lack of estrus, reproductive function decline, and high abortion rate of sows.
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Embodiment 1
[0019] Take 1000kg of this product as an example
[0020] ①Extraction of fairy spleen and Phellodendron cortex
[0021] (1) Accurately weigh 80 kg of Chinese medicine raw material Xianling spleen and 60 kg of Cortex Phellodendri (the following steps are carried out respectively);
[0022] (2) Pulverization: pulverize the weighed traditional Chinese medicine;
[0023] (3) Sieving: after crushing, pass through a 30-mesh sieve;
[0024] (4) Mixing: Mix the sieved Chinese medicine raw materials evenly;
[0025] (5) Extraction: adopt ethanol (75%) extraction reflux method to extract the active ingredients of traditional Chinese medicine;
[0026] (6) Concentration: increase the concentration of ethanol to precipitate the active ingredients;
[0027] (7) drying: after filtering, drying and drying;
[0028] (8) 3150g-4000g of fairy spleen extract and 3050-3600g of Cortex Phellodendri extract can be obtained respectively.
[0029] ②Pre-mixing and mixing
[0030] (1) Other ingre...
Embodiment 2
[0036] Take 1000kg of this product as an example
[0037] ①Extraction of fairy spleen and Phellodendron cortex
[0038] (1) Accurately weigh 80 kg of Chinese medicine raw material Xianling spleen and 60 kg of Cortex Phellodendri (the following steps are carried out respectively);
[0039] (2) Pulverization: pulverize the weighed traditional Chinese medicine;
[0040] (3) Sieve: pass through a 30-mesh sieve after crushing;
[0041] (4) Mixing: Mix the sieved Chinese medicine raw materials evenly;
[0042] (5) Extraction: adopt ethanol (75%) extraction reflux method to extract the active ingredients of traditional Chinese medicine;
[0043] (6) Concentration: increase the concentration of ethanol to precipitate the active ingredients;
[0044] (7) drying: after filtering, drying and drying;
[0045] (8) 3150g-4000g of fairy spleen extract and 3050-3600g of Cortex Phellodendri extract can be obtained respectively.
[0046] ②Pre-mixing and mixing
[0047] (1) Other ingredie...
Embodiment 3
[0053] Animal experiment:
[0054] ①Experimental method
[0055] Take SW female rats (clean grade) 14, 21, 28, 35 days old and 3 months old in the estrous phase of the sexual cycle and 12, 15, 18, 21-24 months old, a total of 9 groups (10 rats in each group) ) at 10:00 to 14:00 by bloodletting, and the hypothalamus, pituitary, and ovary were removed and stored at -70°C, and the levels of estrogen receptor (ER), ER mRNA, and blood E2 were measured. ER was determined by radioligand binding assay (HA P adsorption separation), ERmRNA was measured by ERcDNA a32 PdATP probe for Northern blot analysis. The 15-, 18-, and 21-24-month-old female mice in the hyposexual period were respectively set up: EB group, intramuscular injection of estradiol benzoate 0.05 μg / 10g body weight on the next day, a total of 10 times; GNC group, according to the indicated dose, daily The diet containing 2% of the compound premixed feed of the present invention was taken orally for a total of 15 days; g...
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