Method for detecting malaytea scurfpea fruit in malaytea scurfpea fruit containing preparations

A detection method, the technology of psoraleae, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of no detection, etc., and achieve the effects of eliminating miscellaneous peaks, flat liquid phase baseline, and accurate and stable results

Inactive Publication Date: 2009-06-17
BEIJING TONGRENTANG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] But so far there is no better method that can accurately, effectively and conveniently detect the content of psoralen and isopsoralen in the active ingredients of psoralen in Chinese patent medicines

Method used

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  • Method for detecting malaytea scurfpea fruit in malaytea scurfpea fruit containing preparations
  • Method for detecting malaytea scurfpea fruit in malaytea scurfpea fruit containing preparations
  • Method for detecting malaytea scurfpea fruit in malaytea scurfpea fruit containing preparations

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1. The quality control method of psoralen in compound eucommia strong waist wine

[0088] 1. Instruments and reagents:

[0089] Instrument: SHIMADZU LC-10AD liquid chromatograph; SPD-M10A detector;

[0090] Chromatographic column: DIAMOSILTMC18 (250mm×4.6mm, 5m);

[0091] Reagents: methanol—chromatographically pure (Tianjin Siyou Biomedical Technology Development Company); water—high-purity water (provided by the National Center for Standard Materials), and the rest of the reagents are analytically pure;

[0092] Reference substance: psoralen reference substance (provided by China Institute for the Control of Biological Products, batch number 0739-200108); isopsoralen reference substance (provided by China Institute for the Control of Biological Products, batch number 0738-9907); both are used for content determination, The purity is 100%.

[0093] 2. Methods and results:

[0094] Chromatographic analysis conditions:

[0095] Mobile phase: methanol-water (4...

Embodiment 2

[0104] Embodiment two. The content assay method of psoralen and different psoralen in the compound psoralen tincture

[0105] 1. Preparation of the test solution: take 10ml of the sample, extract by shaking with ether, evaporate to dryness, add an appropriate amount of methanol to the residue to dissolve, quantitatively transfer it to a measuring bottle, add methanol to 10ml, shake well, and place in the treated medium. On the neutral alumina column, the neutral alumina column is filled with 100-200 mesh neutral alumina, collect the eluate flowing out after passing through the column, filter it with a 0.45 μm microporous membrane, and take the subsequent filtrate to obtain .

[0106] 2. Preparation of reference substance solution: Accurately weigh psoralen and isopsoralen reference substances that were dried under reduced pressure to constant weight, add methanol to prepare 20 mg / ml of psoralen and isopsoralen respectively solution, that is.

[0107] 3. Chromatographic condi...

Embodiment 3

[0109] Embodiment three. The comparison of the results of compound eucommia strong waist wine control example

[0110] The compound eucommia strong waist wine control example is a liquor obtained by deleting the psoralen composition on the basis of the original formula of the compound eucommia strong waist wine.

[0111] 1. Instruments and reagents: the same as in Example 1.

[0112] Reference substance: psoralen reference substance (provided by China Institute for the Control of Biological Products, batch number 0739-200108); isopsoralen reference substance (provided by China Institute for the Control of Biological Products, batch number 0738-9907); both are used for content determination, The purity is 100%.

[0113] 2. Methods and results:

[0114] Chromatographic analysis conditions:

[0115] Mobile phase: methanol-water (40:60);

[0116] Detection wavelength: 246nm;

[0117] Column temperature: 40°C;

[0118] Flow rate: 1ml / min;

[0119] The number of theoretical p...

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Abstract

The invention provides a detection method of malaytea scurfpea components in a preparation containing the malaytea scurfpea. The method comprises the following steps: a test sample is preliminarily treated by neutral alumina, then test sample solution and reference solution are prepared, high performance liquid chromatography and an ultraviolet detector are adopted for detection, a mobile phase is methanol and water with the volume ratio of 40:40-80, and detection wavelength is 225-275nm. The detection method successfully eliminates miscellaneous peaks in a liquid phase chromatogram, and causes a liquid phase base line to be smoother and results to be more accurate and stable.

Description

technical field [0001] The invention provides a method for detecting active ingredients in medicines, which is a method for detecting effective ingredients in traditional Chinese medicines, in particular to a method for detecting psoraleae components in Chinese medicine preparations. Background technique [0002] Most of the Chinese patent medicine preparations containing psoraleae as the main ingredient commonly used by the state currently lack effective detection methods for the content of active ingredients or index ingredients. Most of these Chinese patent medicines are Chinese patent medicines for nourishing and strengthening or strengthening the body and eliminating pathogenic factors, such as compound Eucommia strong waist wine, Guogong wine, Jianbu Qiangshen pills, hemiplegia recovery pills, ginseng antler pills, ginseng three whip pills, Wenshen Quanlu pills, Suoyang Gujing Pills, Fennel Juhe Pills, Nutmeg Sishen Pills, Qiangyang Baoshen Pills, Qibao Meiran Pills, A...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/54
Inventor 李银刘柏刚王铭文张爽吴炜夏蓉彭鹏张薇
Owner BEIJING TONGRENTANG CO LTD
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